Preparation technology of porphyra haitanensis phycobiliprotein powder
The technology of phycobiliprotein and seaweed is applied in the field of preparation of seaweed phycobili protein powder, and can solve the problems of complicated separation and purification method of phycobiliprotein, high production cost and inability to prepare in large quantities and the like
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[0017] The present invention uses algae laver as raw material to prepare altar laver phycobiliprotein powder, and the preparation steps of the invention are as follows:
[0018] 1. Altar laver cell crushing process
[0019] The laver is first pulverized with a pulverizer, then the pulverized laver powder is soaked in water for 5-10 hours, the soaked material is taken out, and pulverized with ultrasonic waves.
[0020] 2. Preparation of phycobiliprotein crude extract
[0021] Soak the algae cells directly with distilled water or low-salt solution to make them swell, rupture, and release phycobiliproteins. It takes 10 days to soak in distilled water, and 3 to 4 days to soak in low-salt solution.
[0022] Weigh 5.99g laver powder, soak in distilled water, and fully swell. After 12 days, filter with a 74-micron filter cloth, and centrifuge the filtrate at 5500 g for 20 min at 4°C to remove algae residues, and take the purple-red supernatant as the crude phycobiliprotein extract...
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