Preparation technology of porphyra haitanensis phycobiliprotein powder

The technology of phycobiliprotein and seaweed is applied in the field of preparation of seaweed phycobili protein powder, and can solve the problems of complicated separation and purification method of phycobiliprotein, high production cost and inability to prepare in large quantities and the like

Inactive Publication Date: 2012-05-09
吴乐秦
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complexity of the reported phycobiliprotein separation and purification methods, the p

Method used

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  • Preparation technology of porphyra haitanensis phycobiliprotein powder
  • Preparation technology of porphyra haitanensis phycobiliprotein powder

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Embodiment Construction

[0017] The present invention uses algae laver as raw material to prepare altar laver phycobiliprotein powder, and the preparation steps of the invention are as follows:

[0018] 1. Altar laver cell crushing process

[0019] The laver is first pulverized with a pulverizer, then the pulverized laver powder is soaked in water for 5-10 hours, the soaked material is taken out, and pulverized with ultrasonic waves.

[0020] 2. Preparation of phycobiliprotein crude extract

[0021] Soak the algae cells directly with distilled water or low-salt solution to make them swell, rupture, and release phycobiliproteins. It takes 10 days to soak in distilled water, and 3 to 4 days to soak in low-salt solution.

[0022] Weigh 5.99g laver powder, soak in distilled water, and fully swell. After 12 days, filter with a 74-micron filter cloth, and centrifuge the filtrate at 5500 g for 20 min at 4°C to remove algae residues, and take the purple-red supernatant as the crude phycobiliprotein extract...

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Abstract

A preparation technology of porphyra haitanensis phycobiliprotein powder belongs to the processing field of food supplement, and particularly relates to the preparation technology of the porphyra haitanensis phycobiliprotein powder. The invention provides the preparation technology of the porphyra haitanensis phycobiliprotein powder, which is simple and effective, and is applicable to mass production. The preparation steps comprise: porphyra haitanensis cell disruption technology; phycobiliprotein crude extract preparation; and phycobiliprotein purification.

Description

Technical field: [0001] The invention belongs to the processing field of nutritional products, and more specifically relates to a preparation process of Porphyra phycobiliprotein powder. Background technique: [0002] Laver contains a special pigment protein——phycobiliproteins (PBP), which is an important photosynthetic pigment protein complex existing in red algae, blue-green algae and cryptophytes, which can efficiently capture light energy Passed to chlorophyll, so that the photosynthesis of algae can occur. The content of phycobiliproteins in algae can reach 25%-28% of dry cell weight, which includes phycoerythrin (phycoerythrin, PE), phycocyanin (phycocyanin, PC), allophycocyanin (allo-phycocyanin, APC) ) and phycoerythrocyanin (phycoery-throcyanin). Phycobiliprotein is a very important raw material for photodynamic drugs. Its unique photosensitizing effect can assist laser cancer treatment, enrich in the lesion, and efficiently generate free radicals and active oxyge...

Claims

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Application Information

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IPC IPC(8): A23J1/00
Inventor 吴乐秦
Owner 吴乐秦
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