Biological deodorization and discoloration method for proteins from silk-reeling silkworm chrysalises

A technology for biological deodorization and silkworm chrysalis protein, which is applied to the protein composition of microorganisms, the protein composition of waste, and the protein composition of plant materials, etc., can solve the problems of secondary protein pollution, incomplete deodorization, complicated procedures, etc. The effect of digestion and absorption rate, reasonable amino acid composition and simple technical process

Inactive Publication Date: 2010-12-29
JIANGSU UNIV OF SCI & TECH
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Problems solved by technology

There are many studies on the deodorization of silkworm chrysalis protein, mainly including: First, Qiao Mingheng and Comrade Li Defang published the paper "Development of Deodorized Silkworm Chrysalis Powder by Microbial Method" in the first issue of "Jiangsu Food and Fermentation" in 1997, reporting the use of microorganism Rhizopus oligosporus The method for removing the odor of silkworm chrysalis has simple deodorization process, low cost, obvious deodorization effect, no harmful substance residue, and has great industrial application prospects. However, this method has certain defects, such as: the fermented silkworm chrysalis protein contains microbial metabolism The unique fishy smell substances produced, the protein color is deep, etc.; the second is Zheng Shiyuan, Xie Zhigang, Comrade Cao Youming published in "Food Science and Technology" in 2003 (8) period "one-step degreasing, decolorization and deodorization process research of silkworm chrysalis" , reported the use of gasoline, benzene, ether, acetone and other organic solvents to deodorize silkworm chrysalis protein. The deodorization effect of this method is obvious, but the residual organic solvent in the protein product is high, which affects the food safety of silkworm chrysalis protein; the third is Wu Jian One, Comrade Wei Kemin published the paper "Preparation Method of Low Fat and Odorless Silkworm Chrysalis Protein Powder" in the "Sericulture Science" No. (3) issue in 2003, and reported the use of oxidation deodorization methods such as chlorine, ozone and sodium hypochlorite. It is cumbersome, the deodorization effect is poor, and it will cause secondary pollution of protein, etc.; the fourth is that Yang Jiyuan, Ye Mingqiang, Kuang Zheshi, Liao Sentai, Xiao Gengsheng, Luo Guoqing, Yang Jinbo, and Comrades Zhao Xiangjie published in the fourth issue of "China Sericulture" in 2009. Processing technology of silkworm chrysalis for feed and its application in animal husbandry and aquaculture", reported the use of activated clay and composite fiber for physical adsorption deodorization methods, but the deodorization method was not complete and the protein loss was serious
Although there are many reports about the deodorization of silkworm chrysalis, a method that can remove the peculiar smell of silkworm chrysalis, has a simple processing technology, and is easy to industrialized production has not yet been reported.

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  • Biological deodorization and discoloration method for proteins from silk-reeling silkworm chrysalises

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Embodiment Construction

[0024] The biological deodorization and decolorization method of the silkworm chrysalis protein of the present invention is further specifically described below by examples:

[0025] 1. Collect the silkworm chrysalis after silk reeling, dry them naturally or with hot air, and crush them through a 40-mesh sieve with an ordinary pulverizer.

[0026] 2. Microbial fermentation: take silkworm chrysalis powder and glucose with a weight ratio of 20:1, add 20 times the volume of water, sterilize at 121°C for 30 minutes, cool at room temperature, inoculate Candida tropicalis, and the inoculum size is 20 % (v / w), fermented at 35°C for 38-42 hours.

[0027] 3. Washing with ethanol: adjust the pH value of the fermentation product to 3.5-4.5 with 1mol / L hydrochloric acid, and after standing for 30 minutes, centrifuge (3000rpm, 10min) to collect the precipitate; then use 2 times the volume of 95% (v / v) Soak in ethanol at 50°C for 20 minutes, centrifuge (3000rpm, 10min) to remove ethanol, a...

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Abstract

The invention discloses a biological deodorization and discoloration method for proteins from silk-reeling silkworm chrysalises, which comprises the following steps: carrying out high-temperature sterilization on a certain amount of silkworm chrysalis powder, inoculating with a specific microorganism strain, carrying out solid fermentation, carrying out biological transformation processes of blanching, disinfection, degreasing and the like while soaking in ethanol, dehydrating, and crushing to obtain silkworm chrysalis protein powder with good color and no odor. The method has the advantage of convenient process, and is suitable for industrial processing production. The prepared silkworm chrysalis protein has no odor, and the color of the silkworm chrysalis powder is changed from puce to offwhite. The deodorized silkworm chrysalis protein has more reasonable amino acid composition, the content of seven essential amino acids is increased by 39-85%, and the content of the silkworm chrysalis protein is increased by 2-3%.

Description

Technical field: [0001] The invention relates to a new method for removing the odor and color of reeled silkworm chrysalis protein by using biological technology, and belongs to the technical field of deep processing of silkworm chrysalis in the food industry. Background technique: [0002] my country is the largest silkworm cocoon producer in the world, with an annual output of more than 700,000 tons of silkworm cocoons and about 150,000 tons of dried silkworm chrysalis. Silkworm chrysalis is a by-product left after silk reeling from silkworm cocoons, and has rich nutritional value. The protein content in silkworm chrysalis is very high, and the content of crude protein in dried silkworm chrysalis is as high as 60%-71.9%. It contains 18 kinds of amino acids, and the composition of essential amino acids is reasonable and balanced. The ratio of essential amino acids is 0.7, which is very consistent with the ideal amino acid pattern recommended by FAO / WHO (the content of esse...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/00
Inventor 桂仲争贾俊强吴琼英
Owner JIANGSU UNIV OF SCI & TECH
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