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Preparation method of selenium-rich and zinc-rich protein peptide

A protein peptide and zinc-rich technology, applied in the field of preparation of selenium-rich zinc-rich protein peptide, can solve the problems of low selenium and zinc content, low extraction efficiency, complex process, etc., to enhance physiological functions, reduce residual content, and enhance physiological functions. The effect of the function

Inactive Publication Date: 2018-09-04
武汉佳阳绿昶食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the production process of protein peptides in the prior art, the extraction efficiency is low, the process is complicated, and the content of selenium and zinc in the prepared protein peptides is low

Method used

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  • Preparation method of selenium-rich and zinc-rich protein peptide
  • Preparation method of selenium-rich and zinc-rich protein peptide
  • Preparation method of selenium-rich and zinc-rich protein peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Preparation of selenium-rich zinc-rich protein peptide

[0015] 1. Prepare experimental materials

[0016] A. Using locally grown Chumai 0805 as the main experimental material;

[0017] B. Exogenous protease: Cellulase, acid protease (200,000 U / g), neutral protease (200,000 U / g), alkaline protease (200,000 U / g) were purchased from Rovism (China) Biology Technology Co., Ltd.

[0018] 2. Experimental method

[0019] (1) Cultivation of selenium-enriched zinc-enriched malt: use Na-containing 2 SeO 3 200ppm and ZnSO 4 It is a two-element culture solution of 2000ppm, placed in a light incubator, the temperature is controlled at 18°C, and an appropriate humidity is maintained, and cultivated for 5 days. Among them, the ppm concentration (parts per million) is the concentration expressed by the solute mass in parts per million of the total solution mass, also known as the parts per million concentration. Often used in very small concentrations.

[0020] (2) Ho...

Embodiment 2

[0024] Na in embodiment 2 double element solution 2 SeO 3 with ZnSO 4 Exploration of matching ratio

[0025] Experimental group 1: with Na 2 SeO 3 100ppm and ZnSO 4 500ppm two-element culture solution;

[0026] Experimental group 2: with Na 2 SeO 3 100ppm and ZnSO 4 1000ppm two-element culture solution;

[0027] Experimental group 3: with Na 2 SeO 3 100ppm and ZnSO 4 2000ppm two-element culture solution;

[0028] Experimental group 4: with Na 2 SeO 3 100ppm and ZnSO 4 3000ppm two-element culture solution;

[0029] Four groups of wheat were cultivated for comparative experiments, placed in a light incubator, the temperature was controlled at 18°C, and the humidity was maintained for 5 days. The relative germination rate of wheat and the content of organic selenium and zinc were observed and determined, as shown in Table 1. It can be seen from the table that the optimal ratio is Na 2 SeO 3 Concentration is 100ppm, ZnSO 4 The concentration is 1000ppm, the germ...

Embodiment 3

[0032] Na in embodiment 3 double element solution 2 SeO 3 with ZnSO 4 Concentration exploration and verification of cultivated malt rich in organic selenium and organic zinc

[0033] 1. Cultivation of selenium-enriched zinc-enriched malt: use Na 2 SeO 3 50ppm, 100ppm, 150ppm, 200ppm, 250ppm, 300ppm and one-to-one corresponding ZnSO 4 500ppm, 1000ppm, 1500ppm, 2000ppm, 2500ppm, and 3000ppm were configured into 6 kinds of two-element culture solutions with different concentrations, and they were used as experimental groups 1-6 respectively. These 6 groups of wheat were cultivated for comparative experiments, and placed in a light incubator with temperature control. At 18°C, maintain proper humidity, cultivate for 5 days, observe and measure the relative germination rate of wheat and the content of organic selenium and zinc, as shown in Table 2 below. And Na 2 SeO 3 Concentration greater than 200ppm and ZnSO 4 When it is greater than 2000ppm, the inhibitory effect on whea...

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Abstract

The present invention provides a preparation method of a selenium-rich and zinc-rich protein peptide. The preparation method comprises the following steps: screened wheat is placed in a warm double-element solution to be soaked for accelerating germination; the wheat is germinated after cultivating; the double-element solution is a mixed solution of Na2SeO3 and ZnSO4; harvested malts are placed inwarm water for enzyme deactivating; the enzyme deactivated malts are subjected to tissue homogenizing and wall breaking; cellulose is added for hydrolysis; alkaline protease is added for hydrolysis;the enzymes are inactivated; the pH is adjusted to be neutral; a malt extract is prepared; the malt extract is centrifuged to remove impurities; and then concentrating and spray drying are conducted to obtain the selenium-rich and zinc-rich protein peptide. The used wheat malts have a characteristic of gathering two elements and convert toxic inorganic selenium and zinc into non-toxic organic selenium and zinc, increasing the content of the organic selenium and zinc. The malts are subjected to beating, homogenizing, wall-breaking, double-enzyme hydrolyzing, centrifuging, impurity-removing, concentrating and drying to obtain the selenium-rich and zinc-rich protein peptide. The new method and new technology for obtaining plant nutrients is innovatively opened up.

Description

technical field [0001] The invention relates to the technical field of product processing, in particular to a method for preparing a selenium-enriched zinc-enriched protein peptide. Background technique [0002] my country has very rich plant protein resources. Using high-quality plant protein raw materials to produce a series of active peptides with significant physiological activities is a very potential direction for the high value-added utilization of plant protein intensive processing in my country. Peptides are hydrolysis products of proteins, ie structural fragments of proteins. Two amino acids are linked by peptide bonds to form dipeptides, three amino acids are linked by peptide bonds to form tripeptides, and multiple amino acids are linked by peptide bonds to form polypeptides. Generally, peptides consisting of 2-10 amino acid residues are called oligopeptides. Because peptide molecules are much smaller than protein molecules, but their biological activity is ver...

Claims

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Application Information

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IPC IPC(8): A23J1/00
CPCA23J1/00
Inventor 陈可夫游光福陈涛陈晗杨华山李正锋
Owner 武汉佳阳绿昶食品有限公司
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