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Method for extraction of selenoprotein from selenium-rich tea leaves

A technology for tea leaves and selenoproteins, which is applied in the field of extracting selenoproteins, can solve the problems of insignificant extraction effect and the like, and achieve the effects of simple and easy operation, improved extraction rate and environmental friendliness.

Inactive Publication Date: 2015-03-11
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ultrasonic-assisted extraction has the advantages of short extraction time, high extraction efficiency, and less pollution. However, depending on the extract, sometimes the extraction effect is not obvious compared with the common method.

Method used

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  • Method for extraction of selenoprotein from selenium-rich tea leaves
  • Method for extraction of selenoprotein from selenium-rich tea leaves
  • Method for extraction of selenoprotein from selenium-rich tea leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for extracting selenoprotein from selenium-enriched tea leaves, comprising the following steps:

[0033] (1) Crush the selenium-enriched tea leaves through a 100-mesh sieve, add 60% ethanol at a ratio of 1:25 (g:mL), heat in a water bath at 60°C for 30 minutes, and centrifuge at 3,500 rpm for 10 minutes. Repeat step (1) and dry the resulting precipitate Crushed through a 100-mesh sieve;

[0034] (2) The precipitate obtained in step (1) was added to distilled water according to the ratio of solid to liquid 1:7 (g:mL), frozen in a low-temperature refrigerator for 4 hours, heated in a water bath at 60°C for 30 minutes, then frozen in a low-temperature refrigerator for 4 hours, and then cooled at 60°C Heat in a water bath for 30 minutes, repeat this three times;

[0035] (3) Add 0.10mol / L NaOH solution to step (2) according to the ratio of solid to liquid 1:50 (g:mL), heat in a water bath at 70°C for 3.5h, and centrifuge at 3500rpm for 10min;

[0036] (4) The sup...

Embodiment 2

[0038] A method for extracting selenoprotein from selenium-enriched tea leaves, comprising the following steps:

[0039] (1) Crush the selenium-enriched tea leaves through an 80-mesh sieve, add 60% ethanol at a ratio of 1:20 (g:mL), heat in a water bath at 60°C for 30 minutes, and centrifuge at 3,500 rpm for 10 minutes. Repeat step (1) and dry the resulting precipitate Crushed through 80 mesh sieve;

[0040] (2) The precipitate obtained in step (1) was added to distilled water according to the ratio of solid to liquid (1:5 (g:mL), frozen in a low-temperature refrigerator for 3 hours, heated in a water bath at 50°C for 30 minutes, then frozen in a low-temperature refrigerator for 3 hours, and then cooled at 50°C Heat in a water bath for 30 minutes, repeat this three times;

[0041](3) Add 0.10mol / L NaOH solution to step (2) according to the ratio of solid to liquid 1:50 (g:mL), heat in a water bath at 80°C for 4h, and centrifuge at 3500rpm for 10min;

[0042] (4) The supernat...

Embodiment 3

[0044] A method for extracting selenoprotein from selenium-enriched tea leaves, comprising the following steps:

[0045] (1) Crush the selenium-enriched tea leaves through a 90-mesh sieve, add 50% ethanol at a ratio of 1:30 (g:mL), heat in a water bath at 60°C for 30 minutes, and centrifuge at 3,500 rpm for 10 minutes. Repeat step (1) and dry the resulting precipitate Crushed through a 90-mesh sieve;

[0046] (2) The precipitate obtained in step (1) was added to distilled water according to the ratio of solid to liquid 1:10 (g:mL), frozen in a low-temperature refrigerator for 5 hours, heated in a water bath at 70°C for 20 minutes, then frozen in a low-temperature refrigerator for 5 hours, and then cooled at 70°C Heat in a water bath for 30 minutes, repeat this three times;

[0047] (3) Add 0.15mol / L NaOH solution to step (2) according to the ratio of solid to liquid 1:70 (g:mL), heat in a water bath at 80°C for 4h, and centrifuge at 3500rpm for 10min;

[0048] (4) The supern...

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Abstract

The invention discloses a method for extraction of selenoprotein from selenium-rich tea leaves. The method includes: taking selenium-rich tea leaves as the raw materials, subjecting the selenium-rich tea leaves to drying, crushing and sieving, adding ethanol, conducting heating in water bath at 60DEG C for 30min, performing centrifugation to obtain precipitate, repeating the steps, and then carrying out drying, crushing and sieving; adding distilled water into the obtained precipitate, conducting freezing for 3-5h in a low temperature refrigerator, conducting heating in water bath at 50-70DEG C for 30min, then performing freezing in the low temperature refrigerator for 3-5h, conducting heating in water bath at 50-70DEG C for 30min, and repeating the operation three times; adding a NaOH solution, conducting heating in water bath at 70-90DEG C for 3-4h, performing centrifugation under 3500rpm for 10min, repeating the process again on the precipitate, adjusting the pH of the supernatant to 2.5-3.5, carrying out standing, centrifugation and drying to obtain coarse selenoprotein. The method provided by the invention has the advantages of simple preparation process, easy operation and environmental friendliness. The obtained product has high selenoprotein content, and is a reliable source of organic selenium. Thus, the method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of tea deep processing, in particular to a method for extracting selenoprotein from selenium-enriched tea leaves. Background technique [0002] Selenium is one of the essential trace elements for the human body. Modern medical research has proved that selenium has preventive effects on cancer, cardiovascular and cerebrovascular diseases, Keshan disease, Kaschin-Beck disease and other diseases. Due to the uneven distribution of selenium on the earth, it is currently known that more than 40 countries and regions are deficient in selenium to varying degrees, and more than 2 / 3 of my country is deficient in selenium, involving a population of more than 700 million. Directly using inorganic selenium salts to supplement selenium has certain toxicity, and supplementing selenium through the food chain is an important way to solve the problem of selenium deficiency, but the selenium content in natural foods is general...

Claims

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Application Information

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IPC IPC(8): C07K1/14
Inventor 董文宾王洪言王珺
Owner SHAANXI UNIV OF SCI & TECH
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