Tissue culture and rapid propagation method for Cardamine hirsuta

A technology of tissue culture and broken rice chestnuts, which is applied in horticultural methods, botany equipment and methods, gardening, etc., can solve the problems of low yield of wild pot and bottle broken rice chestnuts and cannot meet social needs, and achieve large-scale production, The effect of low production cost and no pollution to the environment

Inactive Publication Date: 2018-08-28
安徽过湾农业科技有限公司
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Problems solved by technology

[0002] Huping broken rice chestnut is a unique wild vegetable in my country. It is distributed in the Huping Mountain area at the junction of Hunan and Hubei. It is the only pure natural organic selenium that can be dissolved in water in the world. It can be matched with any edible raw materials and can be processed to produce seleniu

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  • Tissue culture and rapid propagation method for Cardamine hirsuta

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Embodiment Construction

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] see figure 1 , a kind of tissue culture and rapid propagation method of broken rice chestnut, its method comprises the following steps:

[0026] A, pretreatment and extraction and disinfection of explants;

[0027] B. Inoculating the sterile explants into the induction medium to obtain callus tissue;

[0028] C, the induction of clustered buds;

[0029] D, inoculating the clustered lateral buds obtained in the strong seedling medium to obtain robust ...

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Abstract

The invention discloses a tissue culture and rapid propagation method for Cardamine hirsute. The method comprises the following steps: A, carrying out pretreatment and material drawing and disinfection of an explant; B, inoculating a sterile explant into an induction medium to obtain a callus tissue; C, inducing multiple shoots; D, inoculating the obtained lateral multiple shoots into a strong sprout culture medium so as to obtain robust test tube seedlings; E, carrying out rooting culture; F, carrying out natural acclimation; and G, carrying out tissue culture seedling transplanting. According to the method disclosed by the invention, the probability of progeny variation in the tissue culture process of Cardamine hirsute can be greatly reduced, good shape of a parent is maintained, the adopted plant tissue culture is convenient for artificially controlling culture conditions, so that the influence of the environment on culture rooting percentage is avoided; and moreover, the method islow in production cost and quick in quantity propagation, does not pollute the environment, and can realize large-scale production.

Description

technical field [0001] The invention relates to the technical field of cultivation of broken rice chestnuts, in particular to a method for tissue culture and rapid propagation of broken rice chestnuts. Background technique [0002] Huping broken rice chestnut is a unique wild vegetable in my country. It is distributed in the Huping Mountain area at the junction of Hunan and Hubei. It is the only pure natural organic selenium soluble in water in the world. It can be matched with any edible raw materials and can be processed into selenium-enriched Food and selenium-enriched beverages can be used as selenium additives for health care products, which have strong market prospects and economic value. Due to the low yield of wild pot and bottle crushed catkins, it cannot meet the huge social demand. Therefore, we propose a crushed Tissue culture and rapid propagation method of Michelia chinensis. Contents of the invention [0003] The purpose of the present invention is to provide...

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 樊浪生樊高俊
Owner 安徽过湾农业科技有限公司
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