Method for improving erythromycin production by transforming Saccharopolyspora erythromycetes sace_3980 gene

A technology of Saccharopolyspora erythromycin and erythromycin, which is applied in the field of genetic engineering, can solve the problems such as the lack of research on the regulation genes of the TetR family of secondary metabolism of Saccharopolyspora erythromycin, and the unclear elucidation of the precise mechanism of erythromycin.

Active Publication Date: 2021-01-01
ANHUI UNIVERSITY
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Problems solved by technology

There are 101 TetR family genes in the whole genome of Rhodomycetes Saccharopolyspora. However, there are still few studies on the regulatory genes of the TetR family in the secondary metabolism of Rhodomycetes Saccharopolyspora. SACE_7040, SACE_0012 and genes regulating erythromycin synthesis and metabolism SACE_5599, SACE_3986, SACE_3446, SACE_7301, etc., and the precise mechanism for regulating erythromycin production is still unclear

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  • Method for improving erythromycin production by transforming Saccharopolyspora erythromycetes sace_3980 gene
  • Method for improving erythromycin production by transforming Saccharopolyspora erythromycetes sace_3980 gene
  • Method for improving erythromycin production by transforming Saccharopolyspora erythromycetes sace_3980 gene

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Embodiment 1

[0030] 1.1 Strains, plasmids and growth conditions

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Abstract

The invention discloses a method of modifying Saccharopolyspora erythromycetes SACE_3980 Genetic method for improving erythromycin production, in Saccharopolyspora erythromycetes, deletion of TetR family transcriptional regulatory genes through genetic engineering SACE_3980 , to obtain high-yield erythromycin engineering strains, and use the obtained strains to ferment and produce erythromycin, which can greatly increase the yield, and provide new technical support for industrial production to increase erythromycin yield.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method for improving the yield of erythromycin by modifying the gene of Saccharopolyspora erythromycetes SACE_3980. Background technique [0002] Erythromycin is produced by the secondary metabolism of Saccharopolyspora erythromycetes and is a typical polyketide antibiotic. Its components include erythromycin A (Er-A), erythromycin B (Er-B), erythromycin C (Er-C), erythromycin D (Er-D), erythromycin E (Er-E) and erythromycin F (Er-F), etc. This type of antibiotic has a broad-spectrum antibacterial effect, and its antibacterial spectrum is similar to that of penicillin, and has a strong inhibitory effect on Gram-positive bacteria. Among the various components of erythromycin, erythromycin A is widely used clinically, and its antibacterial activity is the highest, while chemical derivatives of erythromycin series (clarithromycin, azithromycin, roxithromycin, telithro...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/62C12N1/15C12R1/645
CPCC07K14/37C12P19/62
Inventor 张部昌任少华汪焰胜吴杭王春宇王浩高娟子
Owner ANHUI UNIVERSITY
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