Method for increasing erythromycin fermentation titer

Abstract

The invention relates to a method for increasing erythromycin fermentation titer. The method is characterized in that during a process for fermentation production of erythromycin by taking Saccharopolyspora erythraea as an original strain, an ammonium sulfate solution is slowly added. According to the invention, the ammonium sulfate solution is fed after fermentation is carried out for 60 hours, due to massive breeding of mycelia at this period, large utilization of a nitrogen source of a base material is carried out, oxygen demand of a broth is large, broth viscosity is large, volume is increased, by slowly feeding the ammonium sulfate solution, broth viscosity is changed, dissolved oxygen is improved, synthesis of components of erythromycin can be adjusted, nitrogen source supplement can be simultaneously carried out, thalline vitality is increased, an erythromycin secretion period is prolonged, and an erythromycin fermentation level can be increased.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for increasing the fermentation titer of erythromycin. Background technique [0002] Erythromycin is a macrolide antibiotic with an antimicrobial spectrum similar to that of penicillin. It is often used clinically for the treatment of patients allergic to penicillin. In recent years, due to the extensive development and use of erythromycin derivatives, the demand for erythromycin continues to increase. [0003] In the prior art, in the process of fermenting and producing erythromycin with Saccharopolyspora rubrum as the starting strain, a mixed nitrogen source and sugar water containing a large amount of late-acting nitrogen source and phosphorus element are added throughout the whole process. This method has the following technical disadvantages : 1) As the mycelium proliferates in large quantities, the volume increases in the middle and later stages, resulting in...

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Problems solved by technology

[0003] In the prior art, in the process of fermenting and producing erythromycin with Saccharopolyspora rubrum as the starting strain, a mixed nitrogen source and sugar water containing a large amount of late-acting nitrogen source and phosphorus element are added throughout the whole process. This method has the following technical disadvantages : 1) As the mycelium proliferates in large quantities, the volume increases in the middle and later stages, resulting in the lack of dissolved oxygen; 2) Due to the slow consumption of late-acting nitrogen sources, the residue at the end of fermentation is relatively large, which affects the extraction effect and the quality of the finished product; 3) Some bacteria The whole process of planting only needs to be supplemented with sugar water, and only a sufficient amount of nitrogen source is added to the base material, which will result in rich nutrition in the early stage, a large number of hyphae, a large amount of nitrogen source consumption, increased viscosity of the fermentation liquid, and increased surface tension of the fermentation liquid. The transfer efficiency decreases, the dissolved oxygen concentration decreases, resulting in the lack of dissolved oxygen in the bacteria, the lack of nitrogen source in the middle and later stages, the premature aging of mycelia, shortened fermentation cycle, and reduced yield

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