Detection method and prevention method of phages specific to saccharopolyspora erythraea

Abstract

The invention relates to a detection method and a prevention method of phages specific to saccharopolyspora erythraea. The detection method comprises the following steps: mixing cultured saccharopolyspora erythraea mycelia into an upper culture medium of a double-layer plate culture medium, and then paving the cultured saccharopolyspora erythraea mycelia on a solidified lower culture medium of thedouble-layer plate culture medium; placing the culture medium in an environment needing to be detected for detection at regular time and fixed locations after being solidified, taking the culture medium back, carrying out constant-temperature culture for 28-34 hours at 35-37 DEG C and 40-45% RH, and observing and recording results. According to the method disclosed by the invention, the existenceof the phages can be intuitively observed, meanwhile the quantity and the sedimentation rate of the phages can be detected. The detection method of the invention is an effective, rapid, simple and convenient detection method for phages specific to saccharopolyspora erythraea. The detection method of the invention is suitable for detecting ambient air and also suitable for detection in a liquid environment. According to the invention, infection and outbreak of phages specific to saccharopolyspora erythraea can be effectively prevented.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a detection method and a prevention method of Saccharomydia rubrum-specific phage. Background technique [0002] Phage is a general term for a class of bacterial viruses that infect microorganisms such as bacteria, fungi, actinomycetes, or spirochetes. As a kind of virus, bacteriophage has some characteristics unique to viruses: the individual is small; it does not have a complete cell structure; it only contains a single nucleic acid. Once it leaves the host cell, the phage can neither grow nor replicate. After a phage individual infects a host cell, it can quickly generate hundreds of progeny phage particles, and each progeny particle can infect a bacterial cell to generate hundreds of progeny phage particles. Therefore, once a bacteriophage breaks out in a fermentation enterprise, it will cause production stoppage and incalculable economic losses. ...

AI Technical Summary

Problems solved by technology

Therefore, once a bacteriophage breaks out in a fermentation company, it will cause production shutdown and incalculable economic losses.

[0003] The reproduction speed of bacteriophage is astonishing, and the individual is too small to be observed by naked eyes. Therefore, it is necessary to find an effective, fast, and easy-to-observe detection method to monitor phage in the production environment and research environment of the enterprise, so as to detect it very early and take timely disinfection, etc. measures to prevent its pollution to the factory environment and laboratories, and completely eliminate the possibility of its outbreak