Molecular inversion probe for detecting YVDD drug-resistant mutation of hepatitis B virus and application of molecular inversion probe

Abstract

The invention belongs to the nucleic acid molecule detection field and relates to a molecular inversion probe technology for detecting YVDD drug-resistant mutation of hepatitis B virus, wherein a nucleotide sequence of the molecular inversion probe is expressed as SEQ ID NO: 8; an application method of the molecular inversion probe comprises the steps of designing a PCR (Polymerase Chain Reaction) amplification primer and establishing a single-base extension system of the molecular inversion probe which is applicable to annealing complementation and single-base extension between the molecular inversion probe and a target sequence; establishing a connection system of the molecular inversion probe which is applicable to a connection reaction of the molecular inversion probe; establishing a circumscribed enzyme digestion system for digesting the molecular inversion probe which is not cyclized; establishing a UNG (uracil-N-glycosylase) digestion system for digesting a cyclized molecular inversion probe as a single strand; establishing a PCR amplification reaction system which is applicable to a PCR amplification reaction taking the single stranded molecular inversion probe as a template; and detecting an amplification product. The method is high in sensitivity, specificity and degree of accuracy, simple to operate and applicable to clinical promotion in the absence of expensive detecting instrument.

Description

technical field [0001] The invention belongs to the field of nucleic acid molecular detection, and relates to a molecular inversion probe technology for detecting hepatitis B virus YVDD drug resistance mutation. Background technique [0002] Hepatitis B virus (HBV) is a kind of pathogenic microorganism that seriously endangers human health. At present, there are about 30 million hepatitis B patients in my country, and more than 150 million carriers. About 50% of liver cirrhosis and 70%-90% of liver cancer patients are caused by chronic HBV infection. Therefore, the chronic infection of HBV has become a serious public health problem in our country. [0003] Antiviral therapy is the key to prevent and control the progression of hepatitis B and related liver diseases. Nucleoside analog therapy is one of the most commonly used anti-HBV treatment options in clinical practice. It can inhibit the activity of HBV DNA polymerase, reduce the synthesis of HBV DNA, and inhibit the rep...

AI Technical Summary

Problems solved by technology

DNA sequencing is limited due to the need for special detection instruments and complex operations

Gene chips can quickly and high-throughput detect drug-resistant gene mutations, but technically require fluorescent markers, and there are still defects such as poor repeatability and difficult standardized judgment of results.

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