Rice bacterial leaf blight resistant protein, and coding gene and application thereof

Abstract

The invention discloses a rice bacterial leaf blight resistant protein, and a coding gene and application thereof. The name of the rice bacterial leaf blight resistant protein is Xa7 protein; the amino acid sequence of the rice bacterial leaf blight resistant protein is shown as SEQ ID NO.1; and the nucleotide sequence of the gene for coding the rice bacterial leaf blight resistant protein is shown as SEQ ID NO.2. The cloning of the Xa7 functional gene is finally completed by constructing a genome BAC library of a rice variety IRBB7, screening the library, sequencing candidate insertion fragments, predicting an AvrXa7 recognition site by a target insertion fragment sequence, and carrying out a series of transgenic function complementation tests and gene knockout tests. The invention provides the sequence of the Xa7 functional gene for the first time; and the Xa7 functional gene can be used for researching a rice bacterial leaf blight resistance mechanism, cultivating a rice variety having disease resistance to rice bacterial leaf blight or other disease-resistant crops, or breeding a rice variety having disease resistance to rice bacterial leaf blight.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a rice bacterial blight resistance protein and its coding gene and application. Background technique [0002] Bacterial blight (Xanthomonas oryzae pv. oryzae (Xoo)) is one of the main diseases of rice in my country and Southeast Asia, which is the main rice producing area in the world, and seriously threatens the safety of rice production. The use of host resistance is an effective measure to control the disease. However, due to the pathogenic variation of pathogenic bacteria, the variety resistance is often lost. The persistence of variety resistance and its mechanism have become a key point in current disease resistance research. A deep understanding of the molecular mechanism of persistent disease resistance will be of great help to rice varieties. Durable resistance, continuous and effective disease control is of great significance [Wu Shangzhong, 1982, "Rice Blight and Its Cont...

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Problems solved by technology

Hopkins et al. showed that Xa7 is a dominant resistance gene directly corresponding to an avirulence gene family [Hopkins et al., 1992, Identification of a family of avirulence genes from Xanthomonasoryzae pv.oryzae. Mol Plant Microbe Interact, 5:451- 459.], Kaji and Ogawa located the gene marker at the 107.5cM position of the RGP map of chromosome 6, and the recombination rate with the G1091 marker was 8% [Kaji R and Ogawa T. Identification of the located chromosome of the resistance gene, Xa-7 ,to bacterial leaf blight in rice.Breed.Sci.,1995,45(Suppl.1):79.], Porter et al. carried out fine positioning on Xa7, but because the rice genome sequencing was not perfect at that time, the target gene region could not be Candidate genes for analysis and prediction [Porter et al., 2003, Development and mapping of markers linked to the rice bacterial blight resistance gene Xa7. Crop Science, 43:1484-1493.]

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