Method for detecting trace amount of bisphenol A based on fluorescence resonance energy transfer

Abstract

Disclosed is a method for detecting a trace amount of bisphenol A based on fluorescence resonance energy transfer. According to the method, a bisphenol A aptamer and a short chain DNA complementing to the bisphenol A aptamer are connected to a fluorescent nanometer material and nanometer gold. Through complementation and pairing of basic groups of two single chains DNA, the fluorescent nanometer material is combined with nanometer gold, and a fluorescence resonance energy transfer effect is produced, wherein fluorescence of the nanometer material is quenched by the nanometer gold. When added into a system, bisphenol A competes with a complementary DNA chain to combine with the bisphenol A aptamer, which results in different combining weights of the two nanometer materials, so that the fluorescence intensity of the system is affected. Bisphenol A is detected through measuring a linear relation between the concentration of bisphenol A and the fluorescence intensity of the system. Under an optimal experiment condition, according to the method, a preferable linear relation is shown when the concentration of bisphenol A is in a range of 0.5-100 ng / mL; the regression equation is Y=430.0X+75095 (R<2>=0.993); and the detection limit is 0.16 ng / mL. It is proved by a labeling and recycling experiment that the method can be used for detecting a real sample.

Description

technical field [0001] The invention relates to a bisphenol A trace detection method based on fluorescence resonance energy transfer. Background technique [0002] Bisphenol A, also known as BPA, is widely used industrially in the synthesis of materials such as polycarbonate (PC) and epoxy resins. Since the 1960s, it has been used to make plastic (milk) bottles, food and beverage (milk powder) cans, etc. Bisphenol A is everywhere, from the inside of mineral water bottles, medical equipment and food packaging. However, studies have shown that PC plastics are easy to hydrolyze at high temperatures, and the higher the temperature, the faster the hydrolysis. The bisphenol A produced by hydrolysis will contaminate the packaged food. If bisphenol A accumulates in the body for a long time, it will lead to adverse effects such as decreased immunity. reaction, especially for infants and young children. In addition, many experiments at home and abroad have proved that bisphenol A i...

AI Technical Summary

Problems solved by technology

[0003] At present, the detection methods of bisphenol A mainly include gas chromatography, high performance liquid chromatography, fluorescence photometry, catalytic kinetic spectrophotometry, etc. However, the above methods have some disadvantages, such as expensive detection equipment, insufficient stability, sensitivity, etc. Not high enough, so it is very important to establish a mature bisphenol A detection system

Images