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Tyrosine phosphatase biosensor as well as detection method and application thereof

A technology of tyrosine phosphatase and biosensor, which is applied in the field of analysis and detection, can solve the problems of poor specificity and low sensitivity, and achieve the effect of good specificity, high sensitivity and good specificity

Active Publication Date: 2020-11-24
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, after research by the inventors, it was found that the traditional tyrosine phosphatase (PTP) analysis method has disadvantages such as low sensitivity and poor specificity in complex biological systems.

Method used

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  • Tyrosine phosphatase biosensor as well as detection method and application thereof
  • Tyrosine phosphatase biosensor as well as detection method and application thereof
  • Tyrosine phosphatase biosensor as well as detection method and application thereof

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Experimental program
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Embodiment approach

[0030] A typical embodiment of the present invention provides a tyrosine phosphatase biosensor, including magnetic beads, peptide-DNA conjugates, DNA templates, and signal probes;

[0031] The peptide-DNA conjugate includes a peptide chain and a first DNA sequence, one end of the peptide chain is connected to the 5' end of the first DNA sequence, and the peptide chain contains phosphorylated tyrosine; the other end of the peptide chain is set to be used for connecting a magnetic beads;

[0032]The DNA template is composed of two second DNA sequences and a third DNA sequence from the 5' end to the 3' end, the third DNA sequence is complementary to the first DNA sequence, and after the DNA template is hybridized with the first DNA sequence and then polymerized capable of forming double-stranded DNA with two nickase recognition sites, the complementary strand of the second DNA sequence being a deoxyribozyme;

[0033] The signal probe is a nucleotide sequence, the two ends of the...

Embodiment

[0058] The connection reaction of the substrate and the magnetic beads coated with streptavidin: The connection reaction of the substrate and the magnetic beads (MBs) was carried out according to the instructions of Invitrogen Company. Add 200 μl of streptavidin-coated magnetic beads (MBs) solution (10 mg / ml) to the centrifuge tube and wash twice with 1× phosphate buffered saline (PBS) (pH 7.4). The supernatant was removed by magnetic separation and the magnetic beads (MBs) were resuspended to 200 μl. Then, 2 μl of peptide-DNA conjugates (100 μmol per liter) and 20 μl of 1×PBS were mixed with 128 μl of washed magnetic beads (MBs) solution, and the mixture was placed on a mixer at Incubate at room temperature for 30 minutes. The mixture was subjected to magnetic separation to remove unbound substrate and washed 5 times with 1X phosphate buffered saline (PBS). Then, the mixture was resuspended with 1× phosphate buffered saline (PBS) to a total volume of 80 microliters, and the...

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Abstract

The invention discloses a tyrosine phosphatase biosensor as well as a detection method and application thereof. The tyrosine phosphatase biosensor comprises magnetic beads, a peptide-DNA conjugate, aDNA template and a signal probe, wherein one end of a peptide chain in the peptide-DNA conjugate is linked to a 5' end of a first DNA sequence, the peptide chain contains phosphorylated tyrosine, theother end of the peptide chain is used for linking the magnetic beads; the DNA template consists of two second DNA sequences and a third DNA sequence from a 5' end to a 3' end, the third DNA sequencecan be complemented with a first DNA sequence, the DNA template is hybridized and polymerized with the first DNA sequence so as to form double-chain DNA with two nickase recognition sites, and complementary chains of the second DNA sequences are dnazyme; and the signal probe is a nucleotide sequence, fluorophores and quenchers are respectively linked to two ends of the nucleotide sequence, and thenucleotide sequence can be complemented with the dnazyme; and the dnazyme is an RNA sequence capable of realizing cracking and complementation in dependent reaction of divalent cations

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and relates to a tyrosine phosphatase biosensor, a detection method and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Tyrosine phosphorylation is precisely regulated by intracellular interactions of tyrosine kinases (PTKs) and tyrosine phosphatases (PTPs) to achieve balance. However, the role of tyrosine phosphatases (PTPs) in regulating tyrosine phosphate balance has been underestimated. Recent studies have shown that protein tyrosine phosphatase 1B (PTP1B) may play a key negative regulatory role in insulin / leptin signal transduction, and its abnormal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/42C12Q1/37C12Q1/6844C12Q1/6825
CPCC12Q1/42C12Q1/37C12Q1/6844C12Q1/6825G01N2333/916G01N2333/96433C12Q2531/119C12Q2565/607C12Q2521/301C12Q2521/101C12Q2521/345C12Q2563/107Y02A50/30
Inventor 张春阳李玥颖孙淑丽王黎娟田小锐
Owner SHANDONG NORMAL UNIV
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