Colloidal gold immunocolorimetry kit for detecting neutrophil gelatinase associated lipocalin (NGAL) and preparation method of kit

Abstract

The invention provides a colloidal gold immunocolorimetry kit for detecting neutrophil gelatinase associated lipocalin (NGAL) and a preparation method of the kit and belongs to the field of in-vitro diagnostic reagents. The kit is used for judging whether the situation of individual acute kidney injury occurs or not. According to the method, colloidal gold particles are adopted as a carrier, a neutrophil gelatinase associated lipocalin (NGAL for short) antibody and the colloidal gold particles are coupled, NGAL protein in a sample makes contact with the coupled gold particles in a corresponding detection device, an antigen-antibody-gold compound is formed, the light absorbance of the gold particles under detection wavelength is changed, the change amount of the light absorbance is positively correlated with the concentration of NGAL protein, and then the purpose of detecting NGAL protein in the sample is achieved. The kit has high analysis sensitivity.

Description

field of invention [0001] The invention belongs to the field of in vitro diagnostic reagents, in particular to a colloidal gold immunocolorimetric assay kit for detecting neutrophil gelatinase-associated lipid (NGAL) technical background [0002] Acute kidney injury (AKI) is a short-term clinical syndrome of decreased renal function. Patients may experience a sharp decline in renal function within hours or days due to medication, trauma, surgery, or other reasons. The main cause of the disease is renal ischemia and reperfusion injury caused by decreased renal perfusion leading to hemodynamically mediated glomerular filtration rate, but no renal parenchymal injury. If the decrease in renal perfusion can be corrected in time, the hemodynamic damage can be reversed and the renal function can be recovered rapidly in a short period of time. Otherwise, if it continues to develop, it will lead to further deterioration of the condition and develop into renal tubular necrosis (ATN)...

AI Technical Summary

Problems solved by technology

However, the two methods of ELISA and adsorption chromatography have many shortcomings such as poor repeatability, cumbersome operation, poor linearity, and low accuracy; while the latex immunocolorimetric method has high detection costs, poor stability, and poor analytical sensitivity. It is prone to precipitation, and the complex formed after the reaction should not be cleaned by the automatic biochemical instrument.

[0005] The purpose of the present invention is to solve many deficiencies in the above two methods, and provide a high-efficiency, fast, repeatable, high-accuracy, high detection linearity, and high analytical sensitivity method for the clinic. The two major technical problems of the self-sedimentation of the marker in the gold reagent and the contamination of the cuvette provide a reliable guarantee for clinical diagnosis and reduce the cost of detection

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