The present invention relates to an NGAL latex immunoturbidimetric detection kit and a preparation method thereof. The kit comprises a reagent R1, a reagent R2, a calibration product, and a quality control product. The reagent R1 comprises a buffer solution, an anti-interference agent, a sensitizer, an electrolyte and a preservative, and the reagent R2 comprises latex microspheres with an NGAL antibody, a buffer solution, a stabilizer and a preservative. Compared with the prior art, according to the technical scheme of the present invention, by using the anti-interference agent in the R1 reagent, the anti-interference ability can be significantly improved, and the applicable population of the neutrophil gelatinase-associated lipocalin assay kit is expanded; and the kit detection provided by the present invention has high sensitivity, high specificity, and good stability of the kit, the NGAL content in urine, plasma and serum can be efficiently detected, and the detection results can bewell correlated with imported reagents.