The invention discloses a frame synchronization device capable of suppressing and measuring Doppler in underwater acoustic communication, relating to underwater acoustic communication equipment. The frame synchronization device is provided with a front-loading module, an analogue-digital conversion module, an operation module and a frame synchronization establishment/Doppler measurement module. The input end of the front-loading module is connected with the signal output end of an underwater acoustic transducer at a receiving end; the analogue-digital conversion module is composed of three paths of AD (analogue-digital) sampling converters, namely, an ADC (Analogue-Digital Converter) 1, an ADC2 and an ADC3 which are controlled by DDS (Digital Direct Frequency Synthesis) chips, namely, a DDS1, a DDS2 and a DDS3, and the input ends of the three paths of AD sampling converters, namely, the ADC1, the ADC2 and the ADC3 are connected with the output end of the front-loading module; the input end of the operation module is connected with the digital signal output ends of the three paths of AD sampling circuits, namely, the ADC1, the ADC2 and the ADC3 after analogue-digital conversion; and the input end of the frame synchronization establishment/Doppler measurement module is connected with the three operation result output ends of the operation module after related processing copying. Doppler effect suppression can be simply and conveniently realized, and Doppler can be measured in underwater acoustic communication.