Automatic instrument for microorganism high-flux culture and large concentration range real-time detection

A technology of concentration detection and real-time detection, which is applied in the field of microbiological laboratory research, can solve problems such as consumption and achieve the effect of high-throughput stirring

Active Publication Date: 2016-08-31
杰灵仪器制造(天津)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0021] The problem to be solved by the present invention is that when cultivating microorganisms, since most microorganisms need to consume oxygen and discharge gas products such as carbon dioxide during the metabolic process, constant shaking of the culture solution can speed up the gas exchange in the culture solution, making the microorganisms Get more oxygen, make the nutrients evenly distributed, and keep the microorganisms in a suspended state, so that the microorganisms are in a more suitable growth environment

Method used

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  • Automatic instrument for microorganism high-flux culture and large concentration range real-time detection
  • Automatic instrument for microorganism high-flux culture and large concentration range real-time detection
  • Automatic instrument for microorganism high-flux culture and large concentration range real-time detection

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0132] Embodiment 1: Using any of the above-mentioned devices to carry out the method for high-throughput cultivation of microorganisms and real-time detection of concentration

[0133] Steps include:

[0134] (1) Determine the temperature of the incubator, that is, the growth temperature of microorganisms, so as to determine whether to use a cooling water circulator or an electric heating plate to control the temperature. Set the incubator temperature, sample information, incubation time, concentration detection interval, brushless motor speed and other parameters on the computer software in advance. After the parameters are set, the incubator starts heating or cooling to reach the preset temperature.

[0135] (2) Add liquid culture medium, sterilized magnetic stirrer and microbial strains to be cultured into the sterilized sample bottle, cover the sample cap equipped with air-permeable membrane and silica gel gasket, and number each sample .

[0136] (3) After the tempera...

Embodiment 2

[0138] Embodiment 2: The influence of different vertical distances between light-emitting diodes and photodiodes on the measurement range

[0139] According to the method of Example 1, for Saccharomyces cerevisiae (Saccharomyces cerevisiae) cultivated for 2-96 hours, when the light-emitting diode and the photodiode are installed in parallel on the same vertical plane, the following different center point distances are tested in real time. The measured value at a wavelength of 850nm is the OD measured by the spectrophotometric method of the control 600 Calibrated by method and converted to OD accordingly 600 range of values.

[0140]

[0141] It can be seen that when the distance between the center points is 6 mm, the concentration of the bacterial suspension can be measured in a large range by using backscattered light, and combined with the transmitted light measurement method (OD 600 Value range 0-0.8), can be in 0-15OD 600 Accurate measurement results can be obtained wi...

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Abstract

The invention provides an automatic instrument for microorganism high-flux culture and large concentration range real-time detection. According to the automatic instrument, a transmission light path and a back scattering light path are combined together and share one light source (a light emitting diodes) a light signal receiver (a photodiode) of the transmission light path is arranged in a direction directly facing the light source; a light signal receiver (another photodiode) of the back scattering light path is arranged on a side same as the light source. When in detection, light is emitted from the light source, the two signal receivers receive light intensity signals (transmission light and back scattering light) simultaneously, the relationship between the light signals of the two signal receivers is compared, an appropriate detection light path is selected to measure the concentration of bacterium suspension, and thus the growth situation of microorganism culture can be accurately obtained. Due to adoption of the transmission light path and the back scattering light path of the concentration detection device, a double-detector composite light path can be constructed, defects of a single detection method is avoided, and the automatic instrument is particularly applicable to concentration detection on a test tube container in high-flux culture. Due to adoption of high-flux liquid stirring and a sample bottle tray transmission device in the automatic instrument, and the synergism of a brushless motor and a stepper motor, stirring and concentration detection on a liquid culture medium are organically combined, full-automatic operation is achieved, and multiple defects of a conventional method are avoided.

Description

technical field [0001] This technology belongs to the field of microbiological laboratory research, and is an automatic instrument capable of realizing high-throughput cultivation of microorganisms and real-time detection of large concentration ranges. Background technique [0002] As a large biological population on the earth, microorganisms have been involved in all aspects of human life, and are widely used in food, medicine, chemical raw materials, biological products, feed, pesticides, environmental protection and other fields, and are now also used in textiles, leather, Petroleum exploration and fermentation, metallurgy, polymer compounds and other fields. [0003] The growth and reproduction of microorganisms is a comprehensive reflection of their own metabolism under the interaction of various internal and external environmental factors. Therefore, the data on growth and reproduction can be used as important indicators for studying various physiological, biochemical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/24C12M1/04C12M1/02C12Q1/06
CPCC12M23/08C12M23/38C12M27/02C12M29/04C12M41/12C12M41/18C12M41/36C12M41/48C12Q1/06
Inventor 万俊蒋佩琪齐一伯
Owner 杰灵仪器制造(天津)有限公司
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