The invention discloses an experimental system for the determination of white rat behavior, which is composed of two parts including a hardware device and a white rat video tracking determination system consisting of system hardware and system software, the hardware device comprises a box body, an animal movement case arranged within the box body, an illumination device arranged on the top of the box body and an outdoor air system connected with the box body; the system hardware comprises a video input unit, an image acquisition card and a computer, wherein, the video input unit is connected with the computer via a video line and is arranged on the top of the box body, the image acquisition card and the system software are installed on the computer, the system software comprises the following modules: image acquisition, trace tracking, background modeling, computation of characteristic data, and formatted display and storage of result. The experimental system implements the acquisition of behavior data of the white rat on conditions of low noise and low illumination, causing no disturbance for normal activity of the white rat and improving accuracy of experimental result; the invention, in combination with the white rat video tracking determination system, performs the determination flexibly and results from diversified test parameters.