The invention discloses a circuit security constraint-considering provincial grid AGC (automatic generation control) unit dynamic optimization scheduling method. The method comprises the following steps of firstly, inputting provincial grid basic data, i.e., acquiring a network structure and related data of a provincial grid, acquiring AGC related data, and setting an evolutionary programming parameter; secondly, randomly generating an initial population of control variables (a regulating instruction and a regulating rate), and performing adaptive mutation operation on antibodies in the population; thirdly, correcting the antibodies according to a regulating instruction constraint, a regulating rate constraint and the minimum continuous climbing time constraint, and comprehensively considering target functions of a CPS1 (control performance standard) index and AGC regulation ancillary service charge, and inequality constraints of tie-line power deviation, system frequency deviation, CPS indexes, unit output and circuit safety, and calculating the adaptive value of each antibody in the population; finally, on the basis of adaptability, evaluating and selecting the antibodies, and performing termination judgment to realize decision on the regulating instruction and the regulating rate of an AGC unit.