The invention discloses a Lloyd mirror interference microscopic scale measuring device. The Lloyd mirror interference microscopic scale measuring device comprises a light source, a lens, a slit device, a plane mirror, a photoelectric conversion device, a data processing module, a lever linkage device and a bottom plate, wherein light emitted by the light source generates interference fringes on an optical screen after passing through the lens, the slit device and the plane mirror a detection head of the photoelectric conversion device is connected with the optical screen, an output end of the photoelectric conversion device is electrically connected with an input end of the data processing module, the plane mirror is installed at the top part of one end of the lever linkage device through a guide device, the bottom part of another end of the lever linkage device is provided with an object stage, and the lever linkage device and the guide device are fixed on the bottom plate. The Lloyd mirror interference microscopic scale measuring device adopts a mechanical lever principle to reflect microscopic scale of an object to be measured in the vertical distance between the plane mirror and the slit device, and has the advantages of simple structure, convenient and rapid measurement, high efficiency, high precision and high practicality.