Microalgae cultivation method and device for controlling nutritive salt replenishment on basis of pH feedback

A feedback control and nutrient salt technology, applied in the field of microalgae cultivation, can solve problems such as low yield and affecting production control

Active Publication Date: 2012-07-11
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the blindness of nutrient supplementation in the existing microalgae culture, affecting production control and low yield. 2 Based on the method of cultivating microalgae, a method for cultivating microalgae based on pH feedback control and supplementing nutrients is provided: supplementing CO by pH feedback 2 Keep the pH value of the culture system near a certain value (set value), and measure the CO of the culture system 2 supplementary amount, using CO 2 The amount of supplementation controls the supplementation of nutrient salts, thereby simplifying the production process, and at the same time realizing the control of pH value, carbon source, and nutrient salts of the culture system, and increasing the algae biomass or product (total lipid, protein, polysaccharide, etc.) content

Method used

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  • Microalgae cultivation method and device for controlling nutritive salt replenishment on basis of pH feedback
  • Microalgae cultivation method and device for controlling nutritive salt replenishment on basis of pH feedback
  • Microalgae cultivation method and device for controlling nutritive salt replenishment on basis of pH feedback

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Embodiment 1

[0070] The microalgal species used is Spirulina platensis (Spirulina platensis, 439, freshwater algae species bank of the Typical Culture Collection Committee of the Chinese Academy of Sciences), and the above-mentioned algae species are used for fed-batch culture based on pH feedback control to add nutrients.

[0071] The composition of culture medium and nutrient salt feeding solution is the same as that of Comparative Example 1.

[0072] The microalgae incubator adopts 1000m 2 The open raceway pool uses an impeller stirring device to drive the flow and mixing of the culture solution, and the thickness of the culture solution layer is 15cm. See Nutrient Control Devices figure 1 , wherein the pH sensor is a commercially available online pH sensor (405-DPAS-SC-S8, Mettler-Toledo Instrument Co., Ltd.), CO 2 The flow metering mechanism is commercially available CO 2 Mass flow controller (D07-11C, Beijing Qixing Huachuang Electronics Co., Ltd.), the control mechanism is an aut...

Embodiment 2

[0079] The microalgae used is Spirulina platensis (Spirulina platensis, 439, freshwater algae species bank of Type Culture Collection Committee, Chinese Academy of Sciences). Fed-batch culture with nutrient supplementation based on pH feedback control was carried out with the above-mentioned algae species.

[0080] The composition of the medium is: sodium bicarbonate 8.4g / L, sodium nitrate 0.5g / L, phosphoric acid 0.1g / L, sodium chloride 1.0g / L, potassium chloride 1.0g / L, magnesium sulfate 0.1g / L, Ferrous sulfate heptahydrate 0.01g / L, disodium EDTA 0.08g / L, calcium chloride 0.04g / L. Nutrient salt feeding liquid is the mixed aqueous solution of sodium nitrate, phosphoric acid and magnesium sulfate, and wherein the concentration of sodium nitrate is 5mol / L, and the concentration of phosphoric acid is 0.216mol / L, and the concentration of magnesium sulfate is 0.017mol / L (sodium nitrate, phosphoric acid , the concentration ratio of magnesium sulfate is determined by the ratio of th...

Embodiment 3

[0087] The microalgal species used was Chlorella sp., 1298, freshwater algae species library of the Type Culture Collection Committee of the Chinese Academy of Sciences, and the above-mentioned algae species were used for fed-batch culture based on pH feedback control to supplement nutrients.

[0088] The composition of the medium is: sodium nitrate 0.1g / L, dipotassium hydrogen phosphate 0.04g / L, magnesium sulfate heptahydrate 0.075g / L, calcium chloride dihydrate 0.02g / L, sodium carbonate 0.02g / L, citric acid 0.006g / L, ferric ammonium citrate 0.006g / L, disodium EDTA 0.001g / L. Nutrient salt feeding liquid 1 is 0.25mol / L sodium nitrate.

[0089] The microalgae incubator adopts a pipeline photobioreactor, the diameter of the pipeline is 8cm, the length is 200m, and the actual culture volume is 1m 3 . See Nutrient Control System figure 1 , wherein the pH sensor is a commercially available online pH sensor (GKF-4-a, Shanghai Shendong Biochemical Sensor Factory). CO 2 The flow ...

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Abstract

The invention relates to the field of microalgae cultivation, particularly a microalgae cultivation method and a device for controlling nutritive salt replenishment on the basis of pH feedback. According to the method, CO2 is replenished through pH feedback, the pH value of a cultivation system is kept at a set value, the CO2 replenishment amount of the cultivation system is calculated, and the nutritive salt replenishment is controlled by utilizing the relationship between the CO2 replenishment amount and the nutritive salt consumption. In the method, the replenishment amount of a nutritive salt fed-batch liquid is calculated through a formula (I), wherein alpha refers to a conversion factor between the nutritive salt consumption and the CO2 replenishment amount, and Delta CO2 refers to the CO2 replenishment amount of the cultivation system. Compared with a traditional method, the method provided by the invention can control the nutritive salt concentration of the cultivation system to fluctuate within a small range, the nutritive salt utilization rate during the cultivation process can be improved, and the cost is saved.

Description

technical field [0001] The invention relates to the field of microalgae cultivation, in particular, the invention relates to a method and a device for cultivating microalgae by supplementing nutrient salts based on pH feedback control. Background technique [0002] Microalgae can use CO 2 , light, water and nutrients for photosynthesis to synthesize organic substances such as proteins, carbohydrates, lipids and pigments, which have been widely used in the fields of food, feed, medicine, fine chemicals and dyes. In recent years, with the depletion of fossil energy such as petroleum and coal, the new energy technology of using microalgae to produce hydrogen, produce oil, and refine olefins has attracted great attention. [0003] In microalgae cultivation, the cost of nutrients accounts for a large proportion of the cost of raw materials. Appropriate nutrient control strategies can increase the utilization rate of nutrients and increase the yield of cells or target products, e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12M1/00C12M1/36C12M1/34C12R1/89
CPCC12M21/02C12M41/34C12M41/26
Inventor 丛威鲍亦璐张东梅温树梅刘明吴霞
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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