63results about How to "Improve the training environment" patented technology

When a culture medium is supplied together with a cell to a container (1) and cells are cultured, a liquid content of the container (1) is stirred by repeating an upward motion step for moving the container (1) to a position higher than the initial position thereof, and a downward motion step for returning the upwardly moved container (1) to the initial position thereof and imparting vibration to the container (1) returned to the initial position. Stirring is thereby performed so that the distribution of cells or the component concentration of the culture medium is made uniform while damage to the cells due to shearing stress thereof is minimized, a good culture environment is maintained, adhesion of cells to an inside wall and excessive aggregation of cells are suppressed by the stirring, and cell proliferation is promoted.

The invention discloses a connecting arm structure for a smart boxing frame. The connecting arm structure comprises the boxing frame and boxing targets arranged on the boxing frame. The boxing targetscomprise the upper boxing target, the middle boxing target and the lower boxing target. Each boxing target is connected with a connecting arm. A connecting base is arranged at the position, corresponding to the connecting arm, of the back side of the boxing frame. The two sides in the connecting base are each provided with a rotating shaft. A movable plate is connected with the tail end of each connecting arm. The upper side and the lower side of each movable plate are each provided with a through hole. The rotating shafts penetrate through the through holes to be perpendicular to the upper surfaces and the lower surfaces of the connecting bases. Torsional springs are arranged on the rotating shafts. One end of each torsional spring is connected with the corresponding movable plate, and the other end of each torsional spring abuts against the outer side of the corresponding connecting base. When the connecting arms swing under force, the torsional springs are used for resetting the connecting arms. The connecting arm structure is beneficial for training a user.

The invention discloses a low-serum culture medium for high-density suspension culture of BHK-21 cells and an application of the low-serum culture medium in proliferation of FMDVs (foot and mouth disease viruses). According to researches on the metabolism level and mutual adjustment of nutritional substances such as glucose and the like in the growth process of the BHK-21 cells, the low-serum culture medium for individual BHK-21 cells is provided and comprises an amino acid part, a vitamin part, a balanced salt part and other additive parts. The culture medium is used for high-density suspension culture of the BHK-21 cells, the effective utilization rate of the nutritional substances by the cells can be greatly increased, the concentration of metabolism byproducts is reduced, and accordingly, the cell culture efficiency is improved; besides, the BHK-21 cells cultured by the culture medium have increased sensitivity for different serums of animals suffering from FMDV, the content of effective antigens 146S of the viruses is substantially increased, the purposes of high cell growth efficiency, cell growth controllability and minimization of the concentration of the metabolism byproducts are achieved, the production efficiency of biological products is higher, the production cost is lower, and the low-serum culture medium has remarkable economic benefits.

The invention discloses a brain-like tissue in-vitro printing and culture system and a brain-like tissue in-vitro printing and culture method. The system comprises a printing and culture module and a control module, wherein the printing and culture module comprises a mechanical hand, a printing spray head box, printing spray heads, a mobile platform and a liquid slot; the control module comprises a computer, a signal acquirer and a control box. According to the brain-like tissue in-vitro printing and culture system disclosed by the invention, an in-vitro brain tissue constructing method which is integrated with printing and culture functions can be used for increasing a survival rate of nerve cells in a printing process and later-stage tissue, preventing the interferences of environmental changes to printed brain-like tissue in a process of transferring to the culture system form the printing system, keeping the integral activity and the function construction of the tissue, the printing spray heads are combined with the mechanical hand to realize hybrid printing of various bracket materials and various cells, and a brain-like tissue gradient structure with a special function is conveniently constructed according to a relationship between each area structure of the brain tissue and the functions; the printing and culture device is provided with culture conditions for brain tissue in-vitro regeneration and a detecting device, and can be used for performing functional evaluation in a process of promoting the brain-like tissue to form.