63results about How to "Improve the training environment" patented technology

The invention discloses a low-serum culture medium for high-density suspension culture of BHK-21 cells and an application of the low-serum culture medium in proliferation of FMDVs (foot and mouth disease viruses). According to researches on the metabolism level and mutual adjustment of nutritional substances such as glucose and the like in the growth process of the BHK-21 cells, the low-serum culture medium for individual BHK-21 cells is provided and comprises an amino acid part, a vitamin part, a balanced salt part and other additive parts. The culture medium is used for high-density suspension culture of the BHK-21 cells, the effective utilization rate of the nutritional substances by the cells can be greatly increased, the concentration of metabolism byproducts is reduced, and accordingly, the cell culture efficiency is improved; besides, the BHK-21 cells cultured by the culture medium have increased sensitivity for different serums of animals suffering from FMDV, the content of effective antigens 146S of the viruses is substantially increased, the purposes of high cell growth efficiency, cell growth controllability and minimization of the concentration of the metabolism byproducts are achieved, the production efficiency of biological products is higher, the production cost is lower, and the low-serum culture medium has remarkable economic benefits.

The invention discloses a brain-like tissue in-vitro printing and culture system and a brain-like tissue in-vitro printing and culture method. The system comprises a printing and culture module and a control module, wherein the printing and culture module comprises a mechanical hand, a printing spray head box, printing spray heads, a mobile platform and a liquid slot; the control module comprises a computer, a signal acquirer and a control box. According to the brain-like tissue in-vitro printing and culture system disclosed by the invention, an in-vitro brain tissue constructing method which is integrated with printing and culture functions can be used for increasing a survival rate of nerve cells in a printing process and later-stage tissue, preventing the interferences of environmental changes to printed brain-like tissue in a process of transferring to the culture system form the printing system, keeping the integral activity and the function construction of the tissue, the printing spray heads are combined with the mechanical hand to realize hybrid printing of various bracket materials and various cells, and a brain-like tissue gradient structure with a special function is conveniently constructed according to a relationship between each area structure of the brain tissue and the functions; the printing and culture device is provided with culture conditions for brain tissue in-vitro regeneration and a detecting device, and can be used for performing functional evaluation in a process of promoting the brain-like tissue to form.