Culture medium for itaconic acid production strain and culturing method thereof

Abstract

The invention discloses a culture medium for an itaconic acid production strain and a culturing method thereof. The strain culture medium comprises bran, rice husks and a malt wort culture medium, wherein the bran and the rice husks are in the mass ratio of 1:0.03-1:0.15; the adding amount of the malt wort culture medium is 0.02 to 0.1 percent of a dry culture medium (the total mass of the bran and the rice husks); the water content of the culture is 60 to 80 percent. The culture medium provided by the invention is good in loosening property and little in accumulated water; meanwhile, during a growth process of hyphae, the breathability is good, which is beneficial to dissipation of heat and water in a culturing process; the spore producing capacity of mouldy bran is promoted; the quality and the quantity of the mouldy bran are improved; the stability of the strain is ensured. The spore number of the strain which is cultured by adopting the culture medium disclosed by the invention can reach 1.5*10<11> in every gram of the dry mouldy bran; when the strain is used for inoculating and fermenting to produce the itaconic acid, the initial glucose concentration of a fermenting culture liquid is 14.0 to 15.0 percent, and the acid yield can reach 9.76g / 100ml or above, the fermenting period is within 48 hours, the residual glucose is 0.35 percent or below, and the product conversion rate can reach 67.30 percent or above.

Description

technical field [0001] The invention relates to the field of strain cultivation, in particular to a culture medium for itaconic acid production strains and a cultivation method thereof. Background technique [0002] The scientific name of itaconic acid is methylene succinic acid and methylene succinic acid, which is an unsaturated dibasic organic acid. It contains unsaturated double bonds, has active chemical properties, can carry out polymerization between itself and other monomers, is easily soluble in water, ethanol and other solvents, and can also perform various addition and esterification reactions, and has a wide range of uses . Itaconic acid is an important raw material for the chemical synthesis industry and chemical production; in addition, itaconic acid is also widely used in the fields of medicine, cosmetic reagents, and water treatment agents. [0003] The international market demand for itaconic acid is large. At present, the production methods of itaconic ac...

AI Technical Summary

Problems solved by technology

In the production of itaconic acid fermentation, Aspergillus is often used as the fermentation strain, and the preparation of Aspergillus bran culture medium generally uses wheat bran. Due to the characteristics of wheat bran itself, during the sterilization process, the bran medium is easy to stick to On the wall of the bottle, it is easy to agglomerate, which will eventually affect the sterilization and cultivation effect, which is not conducive to the dissipation of heat and water and the dredging of oxygen during the cultivation period. The quantity and quality of spores decreased, and the qualification rate of gluten strains was low, which could not meet the inoculation requirements of large-scale fermentation to produce itaconic acid, which brought difficulties to the realization of continuous production, caused waste of equipment and manpower, and increased production costs