Cell culture method and cell culture device

Abstract

When a culture medium is supplied together with a cell to a container (1) and cells are cultured, a liquid content of the container (1) is stirred by repeating an upward motion step for moving the container (1) to a position higher than the initial position thereof, and a downward motion step for returning the upwardly moved container (1) to the initial position thereof and imparting vibration to the container (1) returned to the initial position. Stirring is thereby performed so that the distribution of cells or the component concentration of the culture medium is made uniform while damage to the cells due to shearing stress thereof is minimized, a good culture environment is maintained, adhesion of cells to an inside wall and excessive aggregation of cells are suppressed by the stirring, and cell proliferation is promoted.

Description

technical field [0001] The present invention relates to a cell culture method and a cell culture device for culturing cells or tissues, microorganisms, etc. in an artificial environment. Background technique [0002] In recent years, in fields such as pharmaceutical production, gene therapy, regenerative medicine, and immunotherapy, it is required to efficiently and massively culture cells, tissues, microorganisms, etc. in an artificial environment, and various cell culture devices are known. For example, Patent Document 1 proposes a cell culture apparatus in which a medium bag, a cell recovery bag after culture, a connecting tube, etc. are connected to the cell culture bag to perform cell culture. [0003] In addition, in such a cell culture device, it is desired to maintain a good culture environment by stirring so that the distribution of cells in the contents of the cell culture bag and the component concentration of the medium are uniform in addition to promoting the pr...

AI Technical Summary

Problems solved by technology

[0010] However, as in the devices proposed in Patent Document 2 and Patent Document 3, when the contents of the cell culture bag are stirred by rotation and swing, for example, as Figure 4 As shown, the cells C in the content liquid roll and fall over a long distance on the culture surface of the cell culture bag 100, and there is a concern that the cells may be damaged due to shear stress.

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