Cell culture bottle

Abstract

The invention provides a cell culture bottle, which comprises a bottle body, a partition is horizontally arranged in the bottle body, the partition divides the interior of the bottle body into an upper chamber and a lower chamber, an open upper chamber tube is arranged on the upper part of the side of the upper chamber, an open lower chamber tube is arranged on the upper part of the side of the lower chamber, the open upper chamber tube and the open lower chamber tube are provided with seal covers or air-permeable covers, and a dialysis membrane is arranged on the partition. The cell culture bottle provided by the invention decreases the accumulation of metabolic waste concentration, increases the proliferation rate and viability of cells, and ensures a good cell culture environment, and moreover, the cell culture bottle can prevent operational pollution caused by repeated medium replenishment, and saves the time and energy of laboratory technicians.

Description

technical field [0001] The invention relates to a cell culture bottle, in particular to a cell culture bottle which ensures a good environment for cell culture. Background technique [0002] Existing cell culture In the fields of biology and medicine, various cells need to be cultured in the process of research and treatment. Different cell cultures have different environmental requirements, and the equipment used for cell culture is also different, including culture dishes, Culture flasks, culture test tubes, incubators, etc. The cell culture flasks in the prior art have a simple structure and cannot protect the cultured cells and their environment well. [0003] Biological laboratories generally use cell culture bags when carrying out large-scale culture of suspension cells. Currently, the commonly used cell culture bags on the market require experimenters to manually replenish the medium on a regular basis, which is time-consuming and laborious; while the common experimen...

AI Technical Summary

Problems solved by technology

[0002] Existing cell culture In the fields of biology and medicine, various cells need to be cultured in the process of research and treatment. Different cell cultures have different environmental requirements, and the equipment used for cell culture is also different, including culture dishes, Culture flasks, culture test tubes, incubators, etc. The cell culture flasks in the prior art have simple structures and cannot protect the cultured cells and their environment well

[0003] Biological laboratories generally use cell culture bags when carrying out large-scale culture of suspension cells. Currently, the commonly used cell culture bags on the market require experimenters to manually replenish the medium on a regular basis, which is time-consuming and laborious; while the common experimental suspension cell culture on the market The bottles are double-armed, so fresh medium can only be continuously added to the culture bottle, while waste liquid and metabolites cannot be discharged

In addition, contamination is also likely to occur during the process of adding medium to the culture flask. Once the medium is contaminated, the cells in the cell culture flask will be directly unusable, resulting in loss

Images