Fluidized-bed cell-culture disposable film device filled with sheet-shaped carriers

Abstract

The invention discloses a fluidized-bed cell-culture disposable film device filled with sheet-shaped carriers. The fluidized-bed cell-culture disposable film device comprises a closed bag body, wherein the closed bag body is internally filled with the plurality of sheet-shaped carriers; the two sides of the outer part of the closed bag body are respectively provided with an extending edge seal; the closed bag body is fixed in a biological cell-culture shaking bed by fixing mechanisms on the extending edge seals at the two sides; the top surface of the closed bag body is provided with an upperair inlet, an air outlet, a sampling hole and at least one liquid inlet and liquid outlet; a lower air inlet is formed in the bottom surface of the closed bag body; the upper air inlet, the air outlet, the sampling hole, the liquid inlet, the liquid outlet and the lower air inlet are respectively connected with an aseptic quick joint; each liquid outlet is also internally provided with a partitioning device; the sheet-shaped carriers are intercepted in the closed bag body by the partitioning devices arranged at the liquid outlets to realize separation of cells from the sheet-shaped carriers. The fluidized-bed cell-culture disposable film device disclosed by the invention has the advantages of excellent oxygen-dissolving effect, large final density of cell culture and convenience in industrial amplification of wall-attaching cells and the like.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a disposable membrane device for fluidized bed cell culture filled with a sheet carrier Background technique [0002] Currently, bioreactors for cell culture are mainly divided into fluidized bed bioreactors and fixed bed bioreactors. The fixed bed bioreactor is to carry out cell adherent culture by filling the filler in the middle of a fixed container. The filler is composed of a cell culture carrier, and the cell culture carrier is usually a sheet-shaped fiber carrier with a diameter of 2-11mm. The fixed-bed culture technology of cell adherence on a sheet-like carrier was first established by the American NBS company, but the sheet-like carrier is usually wrapped with a wire mesh and placed in a bioreactor tank, and the cells are in a static culture environment for a long time. It is impossible to suspend the sheet-like carrier. After a long time, the metabolites (harmful...

AI Technical Summary

Problems solved by technology

The fixed-bed culture technology of cell adherence on a sheet-like carrier was first established by the American NBS company, but the sheet-like carrier is usually wrapped with a wire mesh and placed in a bioreactor tank, and the cells are in a static culture environment for a long time. It is impossible to suspend the sheet-like carrier. After a long time, the metabolites (harmful substances) produced by the cells will accumulate in the sheet-like carrier and cannot be discharged. It is difficult to achieve large-scale culture of adherent cells, and it is also difficult to directly sample cells.

[0003] The fluidized bed reactor is a reactor that uses gas or liquid to pass through the granular solid layer to keep the solid particles in a suspended state, and to perform a gas-solid phase reaction process or a liquid-solid phase reaction process. The purpose of the fluidized bed is to make Large bubbles are broken to improve gas-solid contact, reduce back-mixing, and improve reaction effects. However, when culturing cells, cells are easily damaged by large bubbles bursting and exploding. poor growth

[0004] In addition, most of the fixed-bed bioreactors currently on the market are mostly non-sterilized products, with high content of microorganisms, endotoxins and other related components, which have a certain impact on subsequent sterilization and production processes and products; and fixed-bed Most of the bioreactors are made of glass and stainless steel and are used repeatedly, which is easy to cause cross-contamination of different batches. A lot of verification work is required, and the relative cost is high. Extremely inconvenient and requires a lot of labor time

[0005] Existing fluidized bed bioreactors and fixed bed bioreactors are not convenient for industrial scale-up of adherent cells, and the culture density of suspension cells is relatively low

For those skilled in the art, the usual way to increase the culture density of suspended cells is to use such a cell culture device as "a cell culture bag" with a patent number of 201521086949.4, but no technician has thought of using the cell culture bag at present. Combined with sheet-like carriers for adherent cell culture, the final yield of cells can be increased by increasing the production density of adherent cells, thereby overcoming the technical difficulty of large-scale culture of adherent cells in traditional fixed-bed bioreactors

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