Human serum creatinine content detecting reagent and method for resisting calcium dobesilate interference

An anti-calcium dobesilate and detection reagent technology is applied in the field of human serum creatinine content detection reagents against interference of calcium dobesilate, can solve problems such as negative deviation of creatinine determination kits, and is convenient for large-scale promotion and use Convenient, wide linear range effects

Pending Publication Date: 2020-07-10
海丰生物科技(北京)有限公司
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Problems solved by technology

Song Jingfang, Pan Benyou, Wu Xianding et al. (Clinical research on the effect of calcium dobesilate on the detection of serum creatinine by oxidase method [J]. Contemporary Medicine, 2019,25(09):175-177) studied the effect of calcium dobesilate on creatinine In the case of interference in the enzyme method, it was found that calcium dobesilate would cause a negative deviation in the creatinine assay kit (sarcosine oxidase method)

Method used

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  • Human serum creatinine content detecting reagent and method for resisting calcium dobesilate interference
  • Human serum creatinine content detecting reagent and method for resisting calcium dobesilate interference
  • Human serum creatinine content detecting reagent and method for resisting calcium dobesilate interference

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Embodiment 1

[0049] In this example, the human serum creatinine content detection reagent against calcium dobesilate interference was prepared. The detection reagent comprises a first reagent (R1), a second reagent (R2) and a third reagent (R3), wherein,

[0050] R1: aqueous solution, pH value 11.5; concentration of each component: sodium carbonate 1g / L;

[0051] R2: aqueous solution, pH 7.5; concentration of each component: trimethylolmethylaminopropanesulfonic acid 9.4g / L, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methyl Aniline sodium salt 0.2g / L, sarcosine oxidase 40KU / L, creatine kinase 20KU / L, ascorbate oxidase 2KU / L, chloramphenicol 0.3g / L and gentamicin 0.2g / L;

[0052] R3: aqueous solution, pH 7.5; concentration of each component: trimethylolmethylaminopropanesulfonic acid 9.4g / L, 4-aminoantipyrine 0.3g / L, creatinase 100KU / L, peroxidase 2KU / L and sodium azide 1g / L.

Embodiment 2

[0054] Anti-interference detection test

[0055] The calcium dobesilate interference test was carried out to the human serum creatinine content detection reagent prepared in Example 1 and the market control reagent.

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Abstract

The embodiment of the invention discloses a human serum creatinine content detecting reagent for resisting calcium dobesilate interference. The detection reagent comprises a first reagent, a second reagent and a third reagent, wherein the first reagent comprises sodium carbonate. The detection reagent is used for determining human serum creatinine based on an enzyme method, and besides, interference of calcium dobesilate drugs in serum can be avoided. The defect for detecting creatinine by a conventional enzyme method is overcome. The embodiment of the invention further provides a human serumcreatinine content detecting method for resisting calcium dobesilate interference.

Description

technical field [0001] The invention relates to the technical field of human body biochemical detection, in particular to a human serum creatinine content detection reagent and method which are resistant to calcium dobesilate interference. Background technique [0002] Creatinine is the final metabolite of creatine produced within muscle cells. In kidney disease and muscle disease with low renal function, the serum creatinine concentration increases; in muscular dystrophy, the serum creatinine concentration decreases. The excretion of creatinine in urine is directly proportional to the total amount of muscle, and is not affected by food, urine volume, and renal tubular reabsorption, so it can be used for the examination of glomerular filtration function. [0003] In the past, the basic picric acid method (Jaffe method) was often used to measure creatinine. This method belongs to the traditional creatinine detection method, but its disadvantages are low specificity, more int...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/48C12Q1/34C12Q1/28C12Q1/26
CPCC12Q1/485C12Q1/34C12Q1/28C12Q1/26G01N2333/90683G01N2333/9123G01N2333/90235G01N2333/986G01N2333/908C12Q2326/96
Inventor 刘芳芳温志国李岩
Owner 海丰生物科技(北京)有限公司
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