Modifying method of bionic nanopores, bionic nanopores and their application

A biomimetic nano-modification method technology, applied in the direction of material electrochemical variables, etc., can solve the problems of limiting the selection range of polyelectrolytes and the inability to modify the response model, achieving the effect of realizing repeated use, widening the selection range, and ensuring the response effect

Active Publication Date: 2019-03-12
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the polyelectrolyte adsorption method, the charge of the polyelectrolyte must be opposite to the charge on the inner surface of the pore, because the charge of the biomimetic nanopore is generally negative, so the response to the change of the electrochemical environment in the pore is generated by the negative charge. The unit cannot be modified into the nanopore by the polyelectrolyte modification method, which greatly limits the selection range of polyelectrolyte, and many mature response models and response molecules cannot be modified into the inner wall of the pore by the polyelectrolyte adsorption method.

Method used

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  • Modifying method of bionic nanopores, bionic nanopores and their application
  • Modifying method of bionic nanopores, bionic nanopores and their application

Examples

Experimental program
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Effect test

Embodiment 1

[0069] Preparation of asymmetric biomimetic nanopores

[0070] For the polyimide (PI) film bombarded by heavy ions, asymmetric nanopores were prepared by track etching. The etching solution used was a NaClO solution with an available chlorine content of 14%. It is a 1M KI solution, etched at 60°C for 3.5h, and washed with deionized water to obtain a PI film with asymmetric biomimetic nanopores with a large pore size of 300nm.

[0071] Use Ag / AgCl electrode, test -2.0V~2.0V in 0.1M KCl solution and measure its volt-ampere characteristic curve at intervals of 0.2V, calculate the rectification ratio as 28 based on the current at -2.0V and 2.0V, and its volt Safety characteristic curve such as image 3 shown.

Embodiment 2

[0073] Modified asymmetric biomimetic nanopores

[0074] like figure 1 As shown, the PI membrane with asymmetric nanopores prepared in Example 1 was placed in a 5% polydiallyldimethylammonium chloride (PDMC) solution and a 7% poly Between styrene sodium sulfonate (PSS) solutions, the small pore end of the PI membrane faces the PDMC solution side, and the large pore end faces the PSS solution side. Apply a voltage of 1V using a Pt electrode, wherein the platinum electrode in the PSS solution is the negative electrode, and the platinum electrode in the PDMC solution is the positive electrode. After applying an electric field for 400s, the PI film is taken out, and then washed with deionized water to obtain a modified biomimetic nanometer. Pore ​​PI membrane.

[0075] Use Ag / AgCl electrode, test -2.0V~2.0V in 0.1M KCl solution and measure its volt-ampere characteristic curve at intervals of 0.2V, and calculate the rectification ratio as -191 based on the current at -2.0V and 2....

Embodiment 3

[0078] Modified asymmetric biomimetic nanopores

[0079] device reference figure 1 As shown, but the utility polyelectrolyte solution and the paired polyelectrolyte solution are changed, specifically, the PI membrane with asymmetric nanopores prepared in Example 1 is placed in a mass concentration of 2% polyallylamine hydrochloride ( PAAm) solution and a mass concentration of 3% p-aminophenylboronic acid-modified sodium polyacrylate (PAANa-APBA) solution, wherein the large pore end of the PI membrane is placed towards the PAAm solution side, and the small pore end is towards the PAANa-APBA solution side. Use a Pt electrode to apply a voltage of 1V, wherein the platinum electrode in the PAANa-APBA solution is the negative electrode, and the platinum electrode in the PAAm solution is the positive electrode. After applying an electric field for 400s, the PI film is taken out, and then washed with deionized water to obtain a modified PI film. PI membranes with biomimetic nanopor...

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Abstract

The invention provides a modifying method of bionic nanopores, which includes the steps of adding a substrate with bionic nanopores between a utility polyelectrolyte solution and a pairing polyelectrolyte solution; under the induction of an electric field, allowing the utility polyelectrolyte solution and the pairing polyelectrolyte solution to be self-assembled in the bionic nanopores through electric crosslinking, wherein the utility polyelectrolyte solution and the pairing polyelectrolyte solution have opposite charges. The modifying method helps greatly widen the selection range of utilitypolyelectrolytes and is suitable for modifying utility polyelectrolytes having the same charge as that in the pores. The modifying method has the advantages of short modifying time, good responding effect, reversible modification, and reusability of the bionic nanopores. The invention also provides bionic nanopores made via the modifying method and application of the bionic nanopores in the production of nanofluidic devices.

Description

technical field [0001] The invention relates to a bionic nano-channel, in particular to a method for modifying a bionic nano-channel, the prepared bionic nano-channel and an application thereof. Background technique [0002] There are various nanopores in living organisms in nature, which play their own unique roles in the normal progress of life activities. For example, protein channel is a kind of asymmetric substance transport channel with excellent performance, and its complex peptide chain structure makes it have excellent selectivity and responsiveness. After in-depth research and understanding of the structure and function of organisms in nature, scientists try to use comprehensive technologies such as nanotechnology, molecular biology, interface chemistry and statistical physics to simulate the structure and intelligence of living organisms. Research, in order to achieve the purpose of imitating nanopores in living organisms. As a stable channel, artificial nanopor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 闻利平滕云飞江雷
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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