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Renal failure rapid detection triple kit and preparation method and application thereof

A rapid technology for renal failure, applied in the field of biotechnology detection, can solve problems such as single detection index and unstable detection results, achieve high sensitivity, benefit daily treatment, and reduce stress.

Inactive Publication Date: 2015-08-26
GOLDBIOMARKERS DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] For this reason, the technical problem to be solved by the present invention lies in the problem that the reagents used to detect renal failure in the prior art are unstable due to the single detection index, and then provide a comprehensive, fast and simple diagnosis and monitoring of kidney failure. A reagent kit for the occurrence and development of aging, and further discloses its preparation method and application

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  • Renal failure rapid detection triple kit and preparation method and application thereof

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Embodiment 1

[0049] Example 1 Preparation of CysC-NAGL-CRP Triple Kit

[0050] The structure of the reagent card described in this embodiment is as follows: figure 1 As shown, the first detection line 5, the second detection line 6, and the third detection line 7 are formed by CysC, NAGL, and CRP antibody coatings respectively, and the quality control line 8 is formed by an anti-mouse IgG antibody coating. The bonding pad 3 is a colloidal gold pad.

[0051] The main materials for preparing the reagent card in this embodiment include:

[0052] The CysC, NGAL and CRP antibodies and specific paired antibodies can be conventional products in the prior art, and the products of Hangzhou Qitai Biotechnology Co., Ltd. are used in this embodiment;

[0053] Goat anti-mouse IgG antibody: Hangzhou Qitai Biotechnology Co., Ltd.;

[0054] Chlorauric acid: Sigma company;

[0055] Nitrocellulose (NC) membrane: SARTORIUS (Germany), CN140, Sartorius, Germany;

[0056] Bovine serum albumin (BSA), polyet...

Embodiment 2

[0063] Example 2 Preparation of CysC-NAGL-CRP Triple Kit

[0064] The structure of the reagent card described in this embodiment is as follows: figure 1 As shown, the first detection line 5, the second detection line 6, and the third detection line 7 are formed by CysC, NGAL, and CRP antibody coatings respectively, and the quality control line 8 is formed by an anti-mouse IgG antibody coating. The binding pad 3 is an immunofluorescent pad.

[0065] The test material of reagent card described in the present embodiment comprises:

[0066] The CysC, NAGL, CRP antibodies and specific paired antibodies are from Guangzhou Greilin Biotechnology Co., Ltd.;

[0067] EU-fluorescent microspheres: Shanghai Biochemical, excitation wavelength 650nm;

[0068] EDC: sigma company, balance weighing, prepare 2.5mg / mL with PB buffer;

[0069] PB buffer: concentration 0.01M, pH7.4, self-made;

[0070] Nitrocellulose (NC) membrane: SARTORIUS (Germany), CN140;

[0071] Bovine serum albumin (BS...

experiment example 1

[0080] Taking the CysC-NAGL-CRP triple kit of the colloidal gold pad prepared in Example 1 as an example, the detection accuracy of the triple kit according to the present invention was verified.

[0081] Get 12 cases of blood samples from patients with clinically diagnosed renal failure, carry out qualitative detection with the CysC-NAGL-CRP triple kit of the colloidal gold pad described in Example 1, add the blood samples dropwise to the detection port of the kit, and observe three strips after 15 minutes The color depth of the test line is semi-quantitatively judged with the color card. When the colors of the three test lines of CysC-NAGL-CRP are respectively darker than the colors of the positions of CysC 1mg / L, NAGL 100μg / L, and CRP 8mg / L on the corresponding color card When , the test result is positive; otherwise, the test result is negative. The specific test results are shown in Table 1. Compared with the clinical results, the positive detection rate of the kit is 100...

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Abstract

This invention belongs to the field of biotechnology detection, particularly relates to a triple kit for early warning, treatment process and monitoring after treatment of renal failure, and further discloses a preparation method and application thereof. Serum cystatin (CysC), neutrophil gelatinase-associated lipocalin (NAGL) and C reactive protein (CRP) are used as detecting markers. The triple kit uses NGAL-CysC-CRP triple antibody as the markers, can effectively monitor the risk of renal failure, especially plays an unexpected effect of monitoring of acute renal failure of persons subjected to a traffic accident, and is high in diagnosis sensitivity of acute renal failure, and strong in specificity, the clinical monitoring accuracy is as high as 95%, and the triple kit can make nephropathy self testing and monitoring of persons with diabetes possible, is used for kidney disease early warning, can timely prevent deterioration of the patient's condition, and reduces the patient's degree of kidney damage.

Description

technical field [0001] The invention belongs to the field of biotechnology detection, specifically relates to a triple kit for early warning, treatment process and prognosis monitoring of renal failure, and further discloses its preparation method and application. Background technique [0002] Acute kidney injury (AKI) is a continuous process of acute renal failure. AKI widely occurs in diabetics, traffic accident injuries, severe patients, and patients after major surgery. It is divided into risk, injury, failure, loss of renal function and End-stage renal disease. Early diagnosis of acute kidney injury helps to implement protective treatment for patients and reduce mortality. [0003] In the prior art, the biomarkers for clinical diagnosis of acute kidney injury are creatinine, blood urea nitrogen and urine output. Creatinine is converted from creatine in skeletal muscle to creatine phosphate, which is converted in the liver and filtered from the glomerulus. A small part...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/531
CPCG01N33/68G01N33/531G01N33/54306G01N2333/4737G01N2333/8139G01N2800/347G01N2800/50G01N2800/52G01N2800/54
Inventor 胡文波李静
Owner GOLDBIOMARKERS DIAGNOSTICS
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