Neutrophil gelatinase-associated lipocalin (NGAL) assay kit (latex-enhanced immunoturbidimetry)

An immunoturbidimetric and latex-enhanced technology, applied in the biological field, can solve the problems of cumbersome operation, long detection time, and specimen pretreatment, etc., and achieve the effects of simple operation, reliable detection results, reduced cost and detection time

Active Publication Date: 2012-09-19
NANJING NORMAN BIOLOGICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Currently, the only clinically used NGAL detection method is enzyme immunoassay (ELISA), wh

Method used

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  • Neutrophil gelatinase-associated lipocalin (NGAL) assay kit (latex-enhanced immunoturbidimetry)
  • Neutrophil gelatinase-associated lipocalin (NGAL) assay kit (latex-enhanced immunoturbidimetry)
  • Neutrophil gelatinase-associated lipocalin (NGAL) assay kit (latex-enhanced immunoturbidimetry)

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Embodiment example

[0035] The invention discloses a kit for detecting the content of NGAL, and those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications that are obvious to those skilled in the art are deemed to be included in the present invention. The products and applications of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the methods and applications described herein without departing from the content, spirit and scope of the present invention. The technology of the present invention.

[0036] The present invention will be further described below with specific examples.

[0037] 1. Preparation of NGAL reagent R1, i.e. sample buffer

[0038]

[0039] Preparation of calibrators in four NGAL reagents

[004...

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Abstract

The invention relates to a kit for assaying NGAL in serum, and provides an NGAL assay kit suitable for a full-automatic biochemical analyzer and a special protein analyzer. The technical scheme includes that the NGAL assay kit comprises reagent R1, reagent R2 and a calibrator, wherein the reagent R1 is composed of a buffer solution, an accelerator, a surfactant and the balance purified water; the reagent R2 is composed of a buffer solution and latex microspheres combined with anti-human NGAL antibodies; and the calibrator is composed of a buffer solution, a stabilizer, a preservative, a certain amount of pure recombinant human NGAL required by concentration, and the balance purified water. The NGAL content in the serum can be quickly assayed through the reagent combination.

Description

Technical field: [0001] The invention relates to the field of biotechnology, in particular to a kit for measuring the content of neutrophil gelatinase-associated apolipoprotein in blood serum by using latex-enhanced immune turbidimetry. Background technique [0002] Human neutrophil gelatinase-associated lipocalin (neutrophil gelatinase-associated lipocalin, NGAL) molecular weight is about 25kDa, covalently bound to neutrophil gelatinase. NGAL is a growth factor in physiological state, mainly involved in the occurrence and growth of early kidney epithelium. The expression level is low in normal tissues including kidney, lung, stomach and colon epithelial tissues. After renal ischemia-reperfusion injury, the epithelium of the proximal convoluted tubule expresses a large amount of NGAL, which is a recently discovered sensitive biomarker for the early diagnosis of AKI. Urinary NGAL is a sensitive indicator of renal ischemia-reperfusion injury, and its expression level is corr...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/531
Inventor 何仕钊
Owner NANJING NORMAN BIOLOGICAL TECH
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