Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of NGAL (Neutrophil Gelatinase-Associated Lipocalin) to preparation of early diagnostic kit for canine acute kidney injury

A technology for acute kidney injury and early diagnosis, applied in the field of biological marker application, can solve problems such as reports on the relationship between the critical value of NGAL and AKI

Inactive Publication Date: 2019-03-26
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it is reported in the prior art that the level of NGAL is closely related to AKI, there is no report on the relationship between the specific critical value of NGAL and AKI

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of NGAL (Neutrophil Gelatinase-Associated Lipocalin) to preparation of early diagnostic kit for canine acute kidney injury
  • Application of NGAL (Neutrophil Gelatinase-Associated Lipocalin) to preparation of early diagnostic kit for canine acute kidney injury
  • Application of NGAL (Neutrophil Gelatinase-Associated Lipocalin) to preparation of early diagnostic kit for canine acute kidney injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] An application of NGAL in preparing a kit for early diagnosis of canine acute kidney injury.

[0019] The biological marker NGAL is formulated into a standard solution with a concentration of 46.96ng / mL, which can be used to prepare a canine acute kidney injury early diagnosis kit.

Embodiment 2

[0021] In order to study the critical concentration of the above-mentioned NGAL, the following experiments were done:

[0022] (1) Experimental animals

[0023] 14 healthy Beagle dogs, 12-16 months old, half male and half male, weighing 5.1±0.2kg, were purchased from the Experimental Animal Center of Heilongjiang Bayi Agricultural Reclamation University, and were raised under the same conditions for 4 weeks for the experiment. The feeding and management during the whole experiment Conditions remain the same.

[0024] (2) Animal experiments

[0025] 1. Establishment of canine acute kidney injury model

[0026] The concentration of NGAL, Kim-1 and SCr in the plasma of the experimental dogs was measured before the injection of gentamicin as the baseline. In this experiment, the KDIGO standard was used to define acute kidney injury if the SCr level ≥ 1.5 times the baseline level within 7 days. Nephrotoxicity was induced by subcutaneous injection of 20 mg / kg gentamicin. During ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of application of biological markers, and particularly relates to application of NGAL (Neutrophil Gelatinase-Associated Lipocalin) to the preparation of anearly diagnostic kit for canine acute kidney injury. The biological marker NGAL is made into a standard solution with a concentration of 46.96 ng / mL. The NGAL standard solution made in the inventionhas the sensitivity of 0.957 and the specificity of 0.96, and has important meaning to the research and development of the early diagnostic kit for the canine acute kidney injury.

Description

technical field [0001] The invention belongs to the technical field of application of biological markers, in particular to the application of a biological marker NGAL in the preparation of a canine acute kidney injury early diagnosis kit. Background technique [0002] Acute kidney injury (acute kidney injury, AKI) is ubiquitous in acutely ill animals, characterized by a sudden and continuous decline in glomerular filtration rate, the overall mortality rate of affected dogs is about 50%, and the mortality rate is similar to that of The severity of kidney injury is closely related, and the prognosis of canine acute kidney injury is affected by the time of diagnosis and treatment method. One of the main reasons affecting mortality is the lack of early diagnosis of the disease, resulting in the loss of the opportunity for early intervention and treatment of the disease. The reason is that there is a lack of valuable early diagnostic markers for acute kidney injury in dogs. So f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2333/47G01N2800/347
Inventor 郑家三连帅聂静宋军朱婷婷
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com