Method for detecting subaqueous acute biological toxicity using photobacteria

A technology of luminescent bacteria and biotoxicity, which is applied in the direction of chemiluminescence/bioluminescence, biochemical equipment and methods, measurement/inspection of microorganisms, etc., which can solve cumbersome problems and achieve the goals of weakening toxicity, good stability and improving test sensitivity Effect

Inactive Publication Date: 2008-02-27
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

Mercury chloride is a highly toxic substance, and the purchase requir

Method used

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Examples

Experimental program
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Embodiment approach

[0024] 2.1 Preparation of fresh bacterial suspension of luminescent bacteria

[0025] (1) Screen strains: take the purchased luminescent bacterial strains and culture them for 48 hours, pick various bacterial colonies for microscopic examination, and select the desired luminescent bacterial colonies.

[0026] (2) Slant strain culture: Before the measurement, take the luminescent bacteria and pick out the first-generation slant on a fresh slant, and immediately transfer the second-generation slant after culturing at 20±0.5°C for 24 hours, and then culture at 20±0.5°C for 24 hours, and then pick up the second-generation slant The third-generation slant was cultured at 20±0.5°C for 12 hours before use. The amount of each inoculation should not exceed one inoculation loop.

[0027] (3) Cultivation of shake-flask bacterial culture: Take nearly one ring of the third-generation slant strain, inoculate it in a 250-mL Erlenmeyer flask filled with 50 mL of culture solution, and culture...

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Abstract

A method to detect the acute biology toxicity by the photobacterium is to prepare the new photobacterium culture firstly, then to adjust the pH of tested water: the pH is 4.5 when the Cu density is higher than other metal and organic matter; the pH is 5.4 when the water sample does not contain Cu and other metal density is higher than the organic matter; the pH is 7.0 when the organic matter in water is higher than metal density. The ZnSO4 is as reference toxicity and matching a series of standard solution which are set in tube which is added in the photobacterium culture and detect the relative luminance. The luminance change of test water is expressed by the relative luminance. The computing formula is: the sample relative luminance = sample tube luminance/reference tube luminance. The invention uses the photobacterium not the toxicity to detect the acute biology toxicity of water.

Description

technical field [0001] The invention relates to a detection method in the field of biotechnology, in particular to a method for detecting acute biological toxicity in water by using luminescent bacteria. Background technique [0002] Luminescent bacteria is a special type of marine bacteria that can survive and emit light in clean water, but its luminescent ability will be weakened when the water quality is polluted. The acute toxicity of water quality organisms can be displayed by using the difference of luminescence intensity of luminescent bacteria in different polluted water bodies. The use of luminescent bacteria to monitor water quality has the characteristics of short time and high sensitivity, and it can also make up for the shortcomings of conventional physical and chemical monitoring, so it is widely used. At present, the traditional luminescent bacteria monitoring methods have problems such as low reproducibility of results, the use of the highly toxic substance ...

Claims

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Application Information

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IPC IPC(8): G01N33/18G01N21/76C12Q1/02
Inventor 白晓慧马良
Owner SHANGHAI JIAO TONG UNIV
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