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Colloidal gold test strip for detecting NGAL (Neutrophil Gelatinase Associated Lipocalin) and preparation method thereof

A colloidal gold test paper and another technology, applied in the field of clinical medical diagnosis, can solve the problems of long detection time, cumbersome operation, and inability to diagnose quickly, and achieve the effect of simple detection method, rapid early prediction, and broad application prospects

Inactive Publication Date: 2012-10-31
GETEIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has certain requirements for detection equipment, cumbersome operation, and long detection time. It cannot quickly and early predict acute renal failure, and cannot be used for rapid bedside diagnosis.

Method used

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  • Colloidal gold test strip for detecting NGAL (Neutrophil Gelatinase Associated Lipocalin) and preparation method thereof
  • Colloidal gold test strip for detecting NGAL (Neutrophil Gelatinase Associated Lipocalin) and preparation method thereof
  • Colloidal gold test strip for detecting NGAL (Neutrophil Gelatinase Associated Lipocalin) and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 detects the preparation of NGAL colloidal gold test paper

[0030] 1. Preparation of colloidal gold-antibody complex

[0031] (1) Preparation of chloroauric acid aqueous solution: Dissolve 10g of chloroauric acid in 1000ml of three-distilled water to prepare a 1% aqueous solution, store it at 4°C for standby, and the validity period is 3 months;

[0032] (2) Preparation of trisodium citrate: Dissolve trisodium citrate in triple distilled water, prepare a 1% aqueous solution, filter through a 0.22μ membrane, store at 4°C for later use, and have a validity period of 7 days;

[0033] (3) Prepare 1% potassium carbonate aqueous solution: dissolve 1g of potassium carbonate in 100ml of triple-distilled water, filter through a 0.22μ membrane, store at 4°C for later use, and have a validity period of 7 days;

[0034] (4) Prepare gold-labeled antibody preservation solution: dissolve 15g of sucrose and 20μl of sodium azide in 100ml of 1% BSA at pH 7.4, filter through...

Embodiment 2

[0050] Example 2 Detecting the use of NGAL colloidal gold test paper

[0051] 1. Qualitative detection

[0052] Drop the sample to be tested on the sample pad of the test paper, when the sample to be tested enters the test end, due to the capillary effect, the liquid moves along the chromatographic direction A. If a purple-red band appears at the position of the quality control line and the detection line of the test paper, it indicates that the test solution contains the corresponding antigen, which is a positive sample; if only the purple-red band appears at the position of the quality control line of the test paper If there is no purple band at the position of the quality control line and the detection line of the test paper, it indicates that the test result is an invalid result. .

[0053] 2. Quantitative detection

[0054] (1) Linear range

[0055] The test strip prepared by the present invention is used to detect the linear range experiment. Take the NGAL standard...

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PUM

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Abstract

The invention discloses a colloidal gold test strip for detecting neutrophil gelatinase associated lipocalin (NGAL) and a preparation method thereof. The colloidal gold test strip comprises a plastic bottom liner, a nitrocellulose membrane, a gold mark pad and a water sucking pad; a quality control line and a detection line which are mutually parallel are arranged on the nitrocellulose membrane; and the quality control line contains a rabbit anti-human IgG antibody, the detection line contains a NGAL (Neutrophil Gelatinase Associated Lipocalin) monoclonal antibody, and the gold mark pad contains another NGAL monoclonal antibody marked by colloidal gold. The colloidal gold test strip has the advantages of being capable of carrying out early prediction on acute renal failure, having strong specificity, being accurate in results, simple in operation and suitable for rapid diagnosis at bedsides.

Description

technical field [0001] The invention belongs to the field of clinical medical diagnosis, and in particular relates to a colloidal gold test strip for detecting neutrophil gelatinase-associated lipocalin (NGAL) and a preparation method thereof. Background technique [0002] Acute renal failure (AFR) secondary to renal tubular cell injury, including ischemic or nephrotoxic injury, remains a common and uncommon practice in clinical medicine and nephrology despite significant advances in supportive care. A potentially devastating problem, the disease is characterized by persistent mortality and high morbidity. Studies have shown that neutrophil gelatinase-associated lipocalin (NGAL), a new member of the lipocalin family, is one of the important markers for the diagnosis of acute kidney injury. US Patent 2004 / 0219603 describes neutrophil gelatinase-associated lipocalin (NGAL) as a urinary biomarker for detecting the early onset of renal tubular cell injury. [0003] In recent y...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/531G01N33/68
Inventor 苏恩本颜彬王勇黄力陈伟
Owner GETEIN BIOTECH
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