A method and kit for detecting the early onset of renal tubular cell injury

Abstract

An early-onset method and kit for detecting renal tubular cell injury, using NGAL as an early urinary biomarker. NGAL is a small secreted polypeptide that is protease resistant and thus readily detected in urine following tubular cell injury. NGAL protein expression was mainly detected in the proximal tubule cells, which resembled a secreted protein with a punctate distribution in the cytoplasm. Apparent NGAL in urine correlates with the amount and duration of renal ischemia and nephrotoxicemia and is a diagnostic feature of tubular cell injury and renal failure. NGAL detection is also a useful marker for monitoring nephrotoxic side effects of drugs or other therapeutic agents.

Description

Background technique

[0001] Acute renal failure (ARF) secondary to renal tubular cell injury, including ischemic or nephrotoxic injury, remains a challenge in clinical medicine and nephrology ( nephrology) with persistently high mortality and morbidity. Decades of previous studies have illustrated the role of persistent vasoconstriction, tubular obstruction, cellular structural and metabolic changes, and inflammatory responses in the pathogenesis of ARF. While these studies have shown possible therapeutic approaches in animal models, efforts to translate them into humans have yielded disappointing results. Reasons for this may include the multifaceted renal response to ischemic injury and nephrotoxins, and the lack of early biomarkers of ARF, resulting in delayed initiation of treatment.

[0002] When the patient's serum creatinine (creatinine) value or: (1) when the baseline serum creatinine level is less than 2.0mg / dL, an increase of at least 0.5mg / dL; (2) when the baselin...

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