Purification method of flavored mogroside V

A mogroside and purification method technology, which is applied to the purification field of mogroside V, can solve the problems of a large proportion of non-glucoside components in solids, incomparable flavor and taste, and inability to remove, and achieves stable and uniform properties. The effect of good sex and simple method

Active Publication Date: 2018-01-26
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the limitation of this method is that it cannot remove the important component that affects the flavor of the Luo Han Guo extract—protein; in addition, the content of mogroside V in the...

Method used

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  • Purification method of flavored mogroside V
  • Purification method of flavored mogroside V

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Polyamide adsorption: Dissolve 1kg of mogroside crude extract I with 8L of water first, and then put the mogroside aqueous solution on a polyamide resin chromatography column at a flow rate of 1BV / h (the mesh number of the polyamide resin is 100 mesh , the dosage is 2kg, the height-to-diameter ratio of the polyamide column is 5:1), and the effluent is collected;

[0048] (2) Alkali adjustment and ultrafiltration: use lye to adjust the pH value of the effluent obtained in step (1) to 10, let it stand for 4 hours, perform coarse filtration, and then use an ultrafiltration membrane with a molecular weight cut-off of 6000D to collect the filtrate;

[0049] (3) Ion exchange adsorption: Put the filtrate obtained in step (2) on a cation exchange resin column (the model of the cation exchange resin is 001×7, the dosage is 2kg, and the ratio of height to diameter of the cation exchange resin column is 6:1) and anion exchange resin column. Exchange resin column (the type of...

Embodiment 2

[0053] (1) Polyamide adsorption: Dissolve 2kg of mogroside crude extract I in 20L of water first, and then put the mogroside aqueous solution on a polyamide resin chromatography column at a flow rate of 0.5BV / h (the mesh size of the polyamide resin is 200 mesh, the dosage is 6kg, the height-to-diameter ratio of the polyamide column is 8:1), and the effluent is collected;

[0054] (2) Alkali adjustment and ultrafiltration: use lye to adjust the pH value of the effluent obtained in step (1) to 10, let it stand for 6 hours, perform coarse filtration, and then use an ultrafiltration membrane with a molecular weight cut-off of 4000D to collect the filtrate;

[0055] (3) Ion exchange adsorption: Put the filtrate obtained in step (2) on the cation exchange resin column (the model of the cation exchange resin is 001×16, the dosage is 7kg, and the height-to-diameter ratio of the cation exchange resin column is 10:1) and the anion exchange resin column. Exchange resin column (the type...

Embodiment 3

[0059] (1) Polyamide adsorption: Dissolve 1kg of mogroside crude extract II in 10L of water first, then put the mogroside aqueous solution on a polyamide resin chromatography column at a flow rate of 2BV / h (the mesh number of the polyamide resin is 150 mesh , the dosage is 2.5kg, the height-to-diameter ratio of the polyamide column is 10:1), and the effluent is collected;

[0060] (2) Alkali adjustment and ultrafiltration: use lye to adjust the pH value of the effluent obtained in step (1) to 9.5, let it stand for 5 hours, perform coarse filtration, and then use an ultrafiltration membrane with a molecular weight cut-off of 5000D to collect the filtrate;

[0061] (3) Ion exchange adsorption: Put the filtrate obtained in step (2) on the cation exchange resin column in turn (the model of the cation exchange resin is 001×8, the dosage is 2.5kg, and the height-to-diameter ratio of the cation exchange resin column is 10:1) and Anion exchange resin column (the type of anion exchan...

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Abstract

A purification method of flavored mogroside V comprises the steps as follows: (1) polyamide adsorption: a mogroside crude extract is dissolved in water, the obtained solution passes a polyamide resinchromatographic column, and an effluent is collected; (2) alkali adjustment and ultrafiltration: the pH value of the effluent is adjusted to range from 8 to 10, the effluent is left to stand and subjected to rough filtration and ultrafiltration, and filtrate is collected; (3) ion exchange adsorption: the filtrate sequentially passes an cation exchange resin column and an anion exchange resin column, and an effluent is collected; (4) decolorization and drying: the effluent passes a decolorizing resin column, an effluent is collected, concentrated and subjected to spray drying, and the flavoredmogroside V is obtained. In the product obtained with the method, the mass content of the mogroside V is larger than 60%, the yield is larger than or equal to 90%, the mass content of an after-bitterness source substance is smaller than 0.06%, the removal rate is larger than 99.5%, and the product has no after-bitterness, tastes fresh and cool and has pure taste, good uniformity and stable property. The method is simple, high in operability and suitable for industrial production.

Description

technical field [0001] The invention relates to a method for purifying mogroside V, in particular to a method for purifying flavored mogroside V. Background technique [0002] Luo Han Guo is rich in Mogroside V, which is more than 300 times sweeter than sucrose. It has a hypoglycemic effect and can be used to assist in the treatment of diabetes; it is also rich in vitamin C, which has anti-aging, anti-cancer and skin-beautifying effects It also has the function of lowering blood fat and losing weight, which can assist in the treatment of hyperlipidemia and improve the image of obese people. Therefore, mogroside V can be widely used in nutritional health products, infant food, puffed food, seasoning, middle-aged and elderly food, solid beverage, pastry, cold food, convenience food, instant food, etc. [0003] At present, the application of mogroside V is becoming more and more extensive, and the development of my country's Luo Han Guo extract industry has begun to take shape...

Claims

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Application Information

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IPC IPC(8): C07J17/00
Inventor 龙伟岸黄华学贺进军刘永胜李伟
Owner HUNAN HUACHENG BIOTECH
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