Extraction method for mogroside

A mogroside and extraction method technology, applied in the directions of steroids, chemical recovery, organic chemistry, etc., can solve the problems of high cost, many processes, complicated operation and the like

Inactive Publication Date: 2012-04-11
WEIHAI TIANHONG AGRI TECH
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Problems solved by technology

Although this method only needs to be separated through the column once, and then another deodorizer is needed to carry out th

Method used

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Experimental program
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Embodiment 1

[0012] 10 kilograms of Luo Han Guo fresh medicinal materials were extracted and concentrated with 60% ethanol (by volume) to obtain 3140 ml of medicinal liquid, and 1750 ml of medicinal liquid (containing mogroside 93537.5 mg) was diluted with deionized water to 1500 ml of HPD-100 macroporous resin on A440=60 , (the volume of the sample liquid is about 60000ml, and the liquid contains 58464mg of mogroside after loading on the column, accounting for 62.5% of the total content of mogroside in the liquid). Impurities, (the washing liquid contains 4209.75 mg mogroside) and then use 25% ethanol (by volume) to wash the resin-adsorbed mogroside until the eluent A238 is less than 0.8 (the eluent contains 22478.38 mg mogroside, accounting for 24% of the total content in the liquid, so far about 91% of the total mogrosides are separated from the liquid), and then use 70% ethanol to elute the remaining active ingredients, combine all the remaining liquids after elution, and obtain after s...

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Abstract

The invention discloses an extraction method for mogroside by taking fructus momordicae as a raw material. The method comprises the following steps of: by utilizing different degrees of capacities of adsorbing other ingredients and the mogroside in fructus momordicae extract by HPD-100 type macroporous resin, loading a sample at a specified speed and washing with ethanol solutions of different concentrations at different elution speeds; combining all eluted remaining liquor; and preparing a mogroside finished product by spray drying. Compared with the prior art in which the extract is subjected to a multiple column-loading adsorption method of adsorption resin and then decolorization resin and/or deodorization resin, the extraction method has the advantages that: a high-purity, decolorized and deodorized mogroside product can be obtained by only an adsorption separation process of strongly polar macroporous adsorption resin without decolorization and deodorization processes. The obtained mogroside has a color which is different from yellowish-brown color and light yellow color of the conventional mogroside, is white or almost white, and has pure sweetness, does not have bitter taste or has the traditional Chinese medicine taste; the content by weight of momordica total glycoside in the mogroside reaches up to over 95 percent; the content by weight of the mogroside V reaches over 65 percent.

Description

technical field [0001] The invention relates to a method for extracting active ingredients from natural plants, in particular to a method for extracting mogroside. Background technique [0002] Luo Han Guo, the fruit of perennial herbaceous vines belonging to Cucurbitaceae, is a unique plant in southern my country. Luo Han Guo has been eaten as traditional Chinese medicine and dried fruit for a long time. It tastes extremely sweet, cool in nature, non-toxic, and enters the spleen and lungs. Quenches thirst, promotes gastrointestinal function, suppresses asthma and lowers blood pressure. Since it was first reported in 1975 that the fruit of Luo Han Guo contained diterpene sweetosides, mogroside IV (mogroside IV), mogroside V, mogroside III, mogroside IIE, mogroside IIIE, 11 kinds of cucurbitane triterpene glycoside compounds such as mogroside VI, mogroside A, and nemogroside, which are the main active substances in the fruit of Luo Han Guo, accounting for about 4% of the we...

Claims

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Application Information

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IPC IPC(8): C07J17/00
CPCY02P20/582
Inventor 毕云英
Owner WEIHAI TIANHONG AGRI TECH
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