Extraction method for mogroside
A mogroside and extraction method technology, applied in the directions of steroids, chemical recovery, organic chemistry, etc., can solve the problems of high cost, many processes, complicated operation and the like
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[0012] 10 kilograms of Luo Han Guo fresh medicinal materials were extracted and concentrated with 60% ethanol (by volume) to obtain 3140 ml of medicinal liquid, and 1750 ml of medicinal liquid (containing mogroside 93537.5 mg) was diluted with deionized water to 1500 ml of HPD-100 macroporous resin on A440=60 , (the volume of the sample liquid is about 60000ml, and the liquid contains 58464mg of mogroside after loading on the column, accounting for 62.5% of the total content of mogroside in the liquid). Impurities, (the washing liquid contains 4209.75 mg mogroside) and then use 25% ethanol (by volume) to wash the resin-adsorbed mogroside until the eluent A238 is less than 0.8 (the eluent contains 22478.38 mg mogroside, accounting for 24% of the total content in the liquid, so far about 91% of the total mogrosides are separated from the liquid), and then use 70% ethanol to elute the remaining active ingredients, combine all the remaining liquids after elution, and obtain after s...
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