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Method for extracting fructus momordicae extract through supercritical fluid

A technology of Luo Han Guo extract and supercritical fluid, which is applied in the production of steroids, bulk chemicals, organic chemistry, etc., can solve the problems of unfavorable decolorization and purification treatment, low content of active ingredients, and deepening of the color of the extract, and achieve the extraction effect Obvious, high extraction rate and short operation cycle

Inactive Publication Date: 2013-12-25
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction methods of Luo Han Guo extract mainly include: (1) water extraction resin separation method; (2) alcohol extraction membrane separation method (including ultrasonic or microwave-assisted extraction); (3) some people use the method of heating extraction, but Excessively high temperature during the extraction process will increase the leached impurities, and the color of the extract will deepen, which is not conducive to the subsequent decolorization and purification treatment
The above methods all have the disadvantages of complex operation, deep color of the obtained extract, strong Chinese medicine flavor and strong after-bitterness, low active ingredient content, large energy consumption, and high production cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Grind the dried fruit of Luo Han Guo, add 30% ethanol aqueous solution of 2 times the weight of Luo Han Guo, then add glucoamylase with a weight of 2‰ of Luo Han Guo, stir evenly, and let stand for 2h; the mixture after standing is washed with supercritical CO 2 Extraction, to obtain the extract containing mogroside, the specific conditions are: CO 2 The flow rate is 8L / h, the extraction pressure is 15MPa, the temperature is 35°C, the pressure in the separation tank is 8MPa, the temperature is 50°C, and the extraction time is 4h; the material is discharged, centrifuged to remove impurities, and the supernatant enters the ADS-17 macropore adsorption Resin, wash the adsorption resin with water until the effluent is colorless, then elute with 50% ethanol solution 8 times the weight of the resin to remove pigment and impurities, collect the ethanol eluate, recover ethanol, and spray dry to obtain a white crystalline powder. As detected by HPLC, the content of mog...

Embodiment 2

[0012] Embodiment 2: Grinding the dried fruit of Luo Han Guo, adding 50% ethanol aqueous solution of 2.5 times the weight of Luo Han Guo, then adding glucoamylase with a weight of 5‰ of Luo Han Guo, stirring evenly, and standing for 3h; 2 Extraction, to obtain the extract containing mogroside, the specific conditions are: CO 2 The flow rate is 10L / h, the extraction pressure is 18MPa, the temperature is 45°C, the pressure in the separation tank is 9MPa, the temperature is 40°C, and the extraction time is 5h; the material is discharged, centrifuged to remove impurities, and the supernatant enters the ADS-7 macropore adsorption Resin, wash the adsorption resin with water until the effluent is colorless, then elute with a 55% ethanol solution 10 times the weight of the resin to remove pigment and impurities, collect the ethanol eluate, recover ethanol, and spray dry to obtain a white crystalline powder. As detected by HPLC, the content of mogroside V was 67.6%.

Embodiment 3

[0013] Embodiment 3: Grinding the dried fruit of Luo Han Guo, adding 40% ethanol aqueous solution of 3 times the weight of Luo Han Guo, then adding glucoamylase with a weight of 4 ‰ of Luo Han Guo, stirring evenly, and standing for 2.5h; 2 Extraction, to obtain the extract containing mogroside, the specific conditions are: CO 2 The flow rate is 9L / h, the extraction pressure is 20MPa, the temperature is 40°C, the pressure in the separation tank is 10MPa, the temperature is 60°C, and the extraction time is 3h; the material is discharged, centrifuged to remove impurities, and the supernatant enters the ADS-5 macropore adsorption Resin, wash the adsorption resin with water until the effluent is colorless, then elute with 60% ethanol solution 9 times the weight of the resin to remove pigment and impurities, collect the ethanol eluate, recover ethanol, and spray dry to obtain a white crystalline powder. As detected by HPLC, the content of mogroside V was 65.3%.

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Abstract

The invention relates to a method for extracting mogroside, in particular to a method for extracting mogroside through supercritical CO2. The method is characterized by comprising the following steps: a, crushing fructus momordicae, adding a certain amount of ethanol aqueous solution, adding saccharifying enzyme accounting for 2-5 per mill of the weight of the fructus momordicae, uniformly stirring and stewing the solution for 2-3 h; b, extracting the crushed fructus momordicae through the supercritical CO2 to obtain extract liquor; and c, discharging, centrifugally removing impurities, decoloring and purifying supernatant through macroporous anion resin with strong basicity, eluting the supernatant through ethanol, recycling the ethanol, and carrying out concentrating, spray drying and crushing treatments to obtain a fructus momordicae extract. As tested by an HPLC (High Performance Liquid Chromatography) method, the weight content of mogroside V in the extract obtained by the extraction process is higher than 65%.

Description

technical field [0001] The invention relates to a method for separating and purifying active ingredients in plants, in particular to a supercritical CO 2 Method of extraction of Luo Han Guo extract. Background technique [0002] Mangosteen, known as the "fairy fruit", is a precious and precious Cucurbitaceae plant unique to my country, and is known as a good medicine and good fruit. It has the functions of clearing away heat and nourishing the lungs, relieving cough, relieving the throat, smoothing the bowel and laxative. The main component of Luo Han Guo extract is mogroside. The active ingredients in mogroside are mainly terpenoids, including glycoside III, glycoside IV, glycoside V, etc. Among them, mogroside V is the main component of its sweetness, and its sweetness is 256~344 times that of sucrose. Due to its combination It has a sugar group and is easily soluble in polar solvents such as water and ethanol, but hardly soluble in organic solvents such as acetone and ...

Claims

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Application Information

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IPC IPC(8): C07J17/00
CPCY02P20/54
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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