Method for extracting high-content mogroside V from dried momordica grosvenori swingle

A technology of mogroside and dried grosvenor fruit, which is applied in the field of biological extraction and can solve problems such as inaccessibility

Inactive Publication Date: 2010-10-20
HILL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the technology of producing mogroside with dried Luo Han Guo as raw material, because of its flavor, taste, color, and content is far from that produced from fresh Luo Han Guo raw materials, it still cannot meet the requirements of domestic and foreign markets

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Extraction: crush 100KG of dried Luo Han Guo and put it into the extraction tank, add 20% ethanol 4 times the weight of the raw material to reflux and extract three times, each time for 2 hours;

[0040] 2. Concentration: Combine three ethanol extracts, recover ethanol, concentrate, to dry fruit: the concentrated solution is 1:4, undergo tubular centrifugal filtration, and take the filtrate as the resin upper column liquid;

[0041] 3. Upper macroporous adsorption resin column: Put the upper column solution into the D-101 macroporous resin column. After entering the liquid medicine, wash it with purified water until it is colorless and transparent, and then wash it with 20% ethanol that is 4 times the volume of the resin. Decolorization, finally desorb with 65% ethanol of 4 times the volume of the resin, and collect the 65% ethanol desorption solution as the decolorization solution;

[0042] 4. Decolorization: Put the decolorized liquid on the activated carbon decolo...

Embodiment 2

[0046] 1. Extraction: crush 100KG of dried Luo Han Guo and put it into the extraction tank, add water 4 times the weight of the raw material to reflux and extract three times, each time for 2 hours;

[0047] 2. Concentration: Combine three water extracts and concentrate to dry fruit: the concentrated solution is 1:4, through tubular centrifugal filtration, and the filtrate is used as the resin upper column liquid;

[0048] 3. Upper macroporous adsorption resin column: Put the upper column solution into the AB-8 macroporous resin column. After entering the liquid medicine, wash it with purified water until it is colorless and transparent, and then wash it with 20% ethanol that is 4 times the volume of the resin. Decolorization, finally desorb with 65% ethanol of 4 times the volume of the resin, and collect the 65% ethanol desorption solution as the decolorization solution;

[0049] 4. Decolorization: put LSA-700 decolorization resin on the decolorization liquid, then elute the ...

Embodiment 3

[0053] 1. Extraction: crush 100KG of dried Luo Han Guo and put it into the extraction tank, add 65% ethanol 4 times the weight of the raw material to reflux and extract three times, each time for 2 hours;

[0054] 2. Concentration: Combine three ethanol extracts, recover ethanol, concentrate, to dry fruit: the concentrated solution is 1:4, through tubular centrifugal filtration, and the filtrate is used as the resin upper column liquid;

[0055] 3. Upper macroporous adsorption resin column: Put the upper column solution into the AB-8 macroporous resin column. After entering the liquid medicine, wash it with purified water until it is colorless and transparent, and then wash it with 20% ethanol that is 4 times the volume of the resin. Decolorization, finally desorb with 65% ethanol of 4 times the volume of the resin, and collect the 65% ethanol desorption solution as the decolorization solution;

[0056] 4. Decolorization: put LSA-700 decolorization resin on the decolorization ...

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Abstract

The invention discloses a method for extracting high-content mogroside V from dried momordica grosvenori swingle. The extraction technique includes the following steps: extraction, concentration, filtration, adsorption, decoloration, concentration and drying, so that the mogroside V product is obtained. The method adopts the dried momordica grosvenori swingle as material for production, the content of the obtained product is high, the taste and the color are good, the adopted equipment required by the technique is simple, continuous production can be carried out, and moreover, because the used solvents are ethanol and water, no pollution is generated.

Description

technical field [0001] The invention relates to a biological extraction method, in particular to a method for extracting high-content mogroside V from dried Luo Han Guo. Background technique [0002] Luo Han Guo is a Cucurbitaceae plant, the dry and mature fruit of Momordica grosvenori Swingle, which is harvested in autumn when the fruit changes from light green to yellow-green. It is a fruit with medicinal effects. Its main component mogroside can be used as both medicine and food sweetener. The main sweet component is mogroside V, which is 300 times sweeter than sucrose , often used as an indicator of content determination in Luo Han Guo extract. [0003] At present, there are many methods and reports for extracting mogroside V, but all of them use fresh Luo Han Guo as raw material, and produce them through water extraction, enzymatic hydrolysis, column purification, water extraction and film concentration extraction, etc. The production is time-consuming and energy-inten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00
Inventor 徐月灿
Owner HILL PHARMA
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