Method for purifying stevioside RC crude extracts

A technology of stevioside and purification method, which is applied in the field of stevioside extraction, can solve problems such as purification methods without RC, and achieve the effect of meeting market demand
CN102321132AInactive Publication Date: 2012-01-18青岛润浩甜菊糖高科有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
青岛润浩甜菊糖高科有限公司
Publication Date
2012-01-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for purifying stevioside RC crude extracts. Mother liquor sugar is prepared into 15 to 20mg / ml feed liquor, the feed liquor flows through large-pore resin absorption columns at the flow speed being 1.5 to 3.0L / min, the large-pore resin absorption columns are benzethylene type nonpolar copolymers and have the average pore size being 5 to 6nm and the pore volume being 1.2 to 1.4 ml / g, and the pH value during the adsorption is 4.0 to 5.0; after the adsorption is completed, ethanol with mass concentration being 75 percent to 80 percent is used for eluting the stevioside absorbed on the resin columns, the elution liquor is collected in a sectional way, a critical point is determined, the elution liquor at the critical point is concentrated at the temperature of 60 to 80 DEG C, obtained solid and liquid are respectively dried, and the crude stevioside is obtained. The stevioside prepared by the method has higher purity, and in addition, the RC content can reach more than 30 percent. The method can adapt to the diversified market requirements of consumers.
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Description

technical field

[0001] The invention relates to a method for extracting stevioside, in particular to a method for purifying the crude extract of stevioside RC. Background technique

[0002] Stevia is widely used in food, beverage, wine making, medicine, cosmetics and other fields after being refined. In recent years, stevia extract, steviol glycoside, has been used as a sweetener. Its calorie energy is very low, and it will not cause adverse reactions to patients with dental diseases and diabetes. It has become an excellent sweetener and has a broad market prospect. Stevioside is the collective name for the extract of stevia rebaudiana. In the current official method of the US FDA, stevioside mainly involves the following 9 components: Stevioside (stevioside), Rebaudioside A (rebaudioside A), Rubusoside, Dulcoside A, Rebaudioside C, Rebaudioside F, Rebaudioside D, Steviolbioside Glycoside), Rebaudioside B (rebaudioside B), stevioside RD is one of the components Rebaudiosi...

Claims

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