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Method for increasing yield of cellulose by adding stevia rebaudiana sugar glucoside

A steviol glycoside and cellulase technology, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problems of high price, low natural yield, difficult application and the like, and achieves low cost and wide raw material sources. , the effect of facilitating large-scale production and application

Active Publication Date: 2015-03-18
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although sophorose exhibits a strong inducing effect, it is difficult to apply in actual production due to its extremely low natural yield and extremely expensive price (about 1200 yuan / mg, product of Sigma Company)

Method used

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  • Method for increasing yield of cellulose by adding stevia rebaudiana sugar glucoside
  • Method for increasing yield of cellulose by adding stevia rebaudiana sugar glucoside
  • Method for increasing yield of cellulose by adding stevia rebaudiana sugar glucoside

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Experimental program
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Effect test

Embodiment 1

[0042] Trichoderma reesei QM9414 was selected as the cellulase producing strain, and the induction effect of steviol glycosides with different additions was analyzed

[0043] Prepare fresh spore liquid of Trichoderma reesei sp.: Streak the Trichoderma strain on a potato medium (PDA) plate and grow at 30 °C for 4-7 days. Using methods known in the art, collect the spores and remove them with a sterile bender. Scrape the surface of the spore-producing culture with a glass rod to scrape off the spores, dilute in sterile water, and determine the spore concentration as 10 7 -10 8 a / mL;

[0044]Get the activated bacterial classification on the plate and inoculate it in a seed medium with a pH value of 4.5. The composition of the seed medium includes:

[0045] Glucose 10g / L, corn steep liquor 10g / L, KH 2 PO 4 15g / L, (NH 4 ) 2 SO 4 5g / L, MgSO 4 ·7H 2 O0.6g / L, CaCl 2 0.5g / L, FeSO 4 ·7H 2 O5mg / L, MnSO 4 ·H 2 O1.6mg / L, ZnSO 4 ·7H 2 O1.4mg / L, CoCL 2 ·6H 2 O0.37mg / L, adju...

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Abstract

The invention relates to a method for producing cellulose by virtue of fermentation and specifically relates to a method for increasing yield of cellulose by adding stevia rebaudiana sugar glucoside. The stevia rebaudiana sugar glucoside is taken as a cellulose fermentation inducer for inducing trichoderma to produce the cellulose. The method for inducing the trichoderma to produce the cellulose by taking the major component stevioside of the stevia rebaudiana sugar glucoside as the cellulose fermentation inducer is wide in raw material source, low in cost and convenient for large-scale production and application.

Description

technical field [0001] The invention relates to a method for producing cellulase by fermentation, in particular to a method for increasing the production of cellulase by adding steviol glycosides. Background technique [0002] Cellulase is a group of enzymes that can hydrolyze cellulose to generate glucose, cellobiose, and cellooligosaccharidase. Cellulase is a compound enzyme mainly composed of three main components, namely endoglucanase, exoglucanase and β-glucosidase, and the three components work synergistically to degrade cellulose macromolecules. With the continuous increase of energy demand and the increasingly serious environmental problems, cellulose raw material, as a renewable resource with abundant reserves and low price, has become a research and development hotspot in the field of biofuels and related high value-added products. The enzymatic hydrolysis of cellulose is one of the main bottlenecks in cellulose biorefinery. The main problem of cellulase hydrolysi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12R1/885
CPCC12N9/2437
Inventor 吕雪峰王敏李建军
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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