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Extraction separation method of salvianolic acid B

A separation method and salvianolic acid technology, applied in the field of bioengineering, can solve problems such as low degradation rate, and achieve the effects of improving stability and shortening the time of enzymatic treatment.

Active Publication Date: 2019-05-28
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to provide an environmentally friendly and efficient extraction and separation method of salvianolic acid B. In the separation process, salvianolic acid B has high stability and low degradation rate, and fully utilizes biotechnology, which is environmentally friendly and efficient

Method used

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  • Extraction separation method of salvianolic acid B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for extracting and separating salvianolic acid B, the steps are as follows:

[0031] (1) Select Danshen medicinal materials: select Danshen medicinal herbal pieces that meet the requirements of the Pharmacopoeia;

[0032] (2) Extraction: Weigh 50 g of Danshen herb decoction pieces according to the weight, add 600 g of water with pH 5.0, add 10 U of xylanase per gram of medicinal material, 1 g of rebaudioside, react at 40 ° C for 3 hours, cool, and filter get filtrate;

[0033] Supplement the liquid, and calculate the extraction rate of salvianolic acid B according to the standard curve to be 3.68%.

[0034] (3) Purification: vacuum concentrate the extract to one-fifth of the original volume, and use macroporous adsorption resin for purification.

[0035] Described step (3) is:

[0036] Soak the resin in 95% ethanol for 18 hours to make it fully swell, choose a glass column, use 95% ethanol to wash the resin column, and wash away the organic impurities contai...

Embodiment 2

[0038] A method for extracting and separating salvianolic acid B, the steps are as follows:

[0039] (1) Select Danshen medicinal materials: select Danshen medicinal herbal pieces that meet the requirements of the Pharmacopoeia;

[0040] (2) Extraction: Weigh 50 g of Danshen herb decoction pieces according to the weight, add 600 parts of water with pH 5.0, add 12 U of xylanase per gram of medicinal material, 1 g of steviol glycoside, react at 45 ° C for 3 hours, cool, and filter get filtrate;

[0041] Supplement the liquid, and calculate the extraction rate of salvianolic acid B according to the standard curve to be 3.61%.

[0042] (3) Purification: vacuum concentrate the extract to one-fifth of the original volume, and use macroporous adsorption resin for purification.

[0043] Described step (3) is:

[0044] Soak the resin in 95% ethanol for 18 hours to make it fully swell, choose a glass column, use 95% ethanol to wash the resin column, and wash away the organic impuriti...

Embodiment 3

[0046] A method for extracting and separating salvianolic acid B, the steps are as follows:

[0047] (1) Select Danshen medicinal materials: select Danshen medicinal herbal pieces that meet the requirements of the Pharmacopoeia;

[0048] (2) Extraction: Weigh 50 g of Danshen herb decoction pieces according to weight, add 600 g of water with pH 5.0, add 10 U of xylanase per gram of medicinal material, 1 g of rebaudioside, 1 g of steviol glycoside, and react at 45°C for 2 hour, cooling, filtering to obtain the filtrate;

[0049] Supplement the liquid, and calculate the extraction rate of salvianolic acid B according to the standard curve to be 3.86%.

[0050] (3) Purification: vacuum concentrate the extract to one-fifth of the original volume, and use macroporous adsorption resin for purification.

[0051] As preferably, said step (3) is:

[0052] Soak the resin in 95% ethanol for 18 hours to make it fully swell, choose a glass column, use 95% ethanol to wash the resin column, ...

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Abstract

The invention discloses an extraction separation method of salvianolic acid B. The method comprises the following steps of (1) radix salviae miltiorrhizae medical material selection: selecting radix salviae miltiorrhizae medical material decoration pieces conforming to the specification of pharmacopeia; (2) extraction: weighing 1 part of radix salviae miltiorrhizae medical material decoration pieces in parts by weight; adding 12 parts of water with the pH being 5.0; adding xylanase, rebaudioside and / or stevioside accounting for 5 to15 U in per g of medical materials; performing reaction for 2to 3 hours at 38 to 45 DEG C; performing cooling and filtering to obtain filter liquid; (3) purification: performing vacuum concentration on the extraction liquid to 1 / 5 original volume; performing purification by macroreticular adsorption resin. The xylanase, rebaudioside and stevioside are comprehensive used, so that the enzymic method processing time can be effectively reduced; in the purification process, the activity of the salvianolic acid B can also be protected, so that the stability of the salvianolic acid B in the purification process can be greatly improved. When the two ingredientsare singly used, the effect can be respectively achieved; after the combination, the cooperated effects are achieved.

Description

technical field [0001] The invention relates to a method for extracting and separating salvianolic acid B, belonging to the technical field of bioengineering. Background technique [0002] Danshen, the name of traditional Chinese medicine. It is the dry root and rhizome of Salvia miltiorrhiza Bge. Excavated in spring and autumn, removed the sediment and dried. Distributed in most parts of the country. Have blood circulation promoting and blood stasis dispelling, stimulate the menstrual flow to relieve pain, clear away heart-fire and relieve restlessness, the effect of cooling blood and eliminating carbuncle. For chest pain and heart pain, epigastric and hypochondriac pain, lump in the abdomen, heat arthralgia pain, vexation and insomnia, irregular menstruation, dysmenorrhea, sore swelling and pain. [0003] The early research on the effective components of Danshen mainly focused on fat-soluble components, and more than 40 compounds have been obtained. These compounds ca...

Claims

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Application Information

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IPC IPC(8): C07D307/84
Inventor 刘东锋于霞
Owner NANJING ZELANG AGRI DEV
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