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Method for refining and purifying steviolbioside

A technology of stevioside and refined products, which is applied in the field of refining and purifying stevioside, which can solve the problems of organic solvent introduction, high energy consumption, insufficient research on stevioside, etc., and achieve the effect of low cost and simple process

Inactive Publication Date: 2017-05-10
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research on steviolbioside (Steviolbioside referred to as SB) is still insufficient. Some studies focus on the use of chemical and enzymatic hydrolysis of steviol glycosides to prepare high-purity SB, which has the disadvantages of bringing in organic solvents and high energy consumption; Part of the research focuses on removing SB from steviol glycosides to prepare highly pure RA
In the patent publication number CN105597843 A, a method for removing steviol glycoside SB by anion exchange resin is reported. This patent reports how to remove SB in stevioside, but there is no report on how to refine and purify SB

Method used

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  • Method for refining and purifying steviolbioside
  • Method for refining and purifying steviolbioside
  • Method for refining and purifying steviolbioside

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Take 100ml of gel-type cation exchange resin and put it into the chromatographic column, and pretreat the resin according to the following steps: use 2BV 4% hydrochloric acid to pass through the cation exchange resin, wash with water until the pH is 6.31; then use 2BV 4% sodium hydroxide aqueous solution to pass The cation exchange resin is washed with water until the pH is 8.72; finally, 2BV of 4% hydrochloric acid is used to pass through the cation exchange resin, and 5BV of water is used for washing. Take 80g rich in SB stevioside (stevia rebaudiana extract) (total glycoside sugar content is 80.54%, of which SB content is 16.68%, see figure 1 ) dissolved in water. Pass the high SB solution through the chromatographic column and collect the effluent, stand at -4°C for crystallization, and filter and separate after 24 hours to obtain SB stevioside (the total glycoside content is 95.03%, of which the SB content is 85.64%, see figure 2 ).

Embodiment 2

[0027] Take 100ml of macroporous cation exchange resin and put it into the chromatographic column, and pretreat the resin according to the following steps: use 2BV 4% hydrochloric acid to pass through the cation exchange resin, wash with water until the pH is 6.75; then use 2BV 4% sodium hydroxide aqueous solution to pass through The cation exchange resin is washed with water until the pH is 8.53; finally, 4BV of 4% hydrochloric acid is passed through the cation exchange resin and 8BV of water is used for washing. Take 150g rich in SB stevioside (stevia rebaudiana extract) (total glycoside sugar content is 64.60%, of which SB content is 34.22%, see image 3 ) dissolved in water. Pass the high SB solution through the chromatographic column and collect the effluent, stir at 24°C at 30r / s to precipitate crystals, and filter and separate after 36h to obtain SB stevioside (the total glycoside content is 91.42%, of which the SB content is 85.31%, See Figure 4 ).

Embodiment 3

[0029] Take 200g rich in SB stevioside (stevia rebaudiana extract) (total glycoside sugar content is 65.18%, of which SB content is 55.21%, see Figure 5 ) dissolved in water. Add 50ml of 4% hydrochloric acid to the high SB solution, stir at 4°C at 40r / s to precipitate crystals, and filter and separate after 12h to obtain SB stevioside (the total glycoside content is 97.75%, and the SB content is 90.81%, see Image 6 ).

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Abstract

The invention discloses a method for refining and purifying steviolbioside, and belongs to the technical field of natural extract refining. The method comprises the following steps: adding water to dissolve stevia rebaudiana to obtain a steviolbioside containing solution, supplementing H<+> into the solution for crystallization, and performing solid-liquid separation to obtain a crystallization product, wherein the crystallization product is a refined steviolbioside product. The method is simple in process and controllable in condition, and a stevioside product with steviolbioside content of 85 percent or higher can be obtained.

Description

technical field [0001] The invention belongs to the technical field of refining natural extracts, in particular to a method for refining and purifying steviolbioside. Background technique [0002] Stevioside is a high-sweet, low-calorie, safe and non-toxic terpene glycoside extracted from the leaves of Stevia rebaudiana, an herb of Compositae, mainly including stevioside (Stevioside referred to as ST), rebaudioside A (Rebaudioside Rebaudioside B (referred to as RB), rebaudioside C (referred to as RC), steviolbioside (referred to as SB) and other glycoside ingredients. Various components in steviol glycosides have different taste, taste, and sweetness due to different molecular structures, and have different applications in food, medicine, and daily chemical industries. The preparation of high-purity RA, RB, and SB has become the focus of subdivided applications. The inevitable trend. Among them, SB, as a component of total steviol glycosides, has the effects of lowering bl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/08
CPCC07H15/256C07H1/08
Inventor 牛志平张亭田洪高伟王艳芳
Owner CHENGUANG BIOTECH GRP CO LTD
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