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A method for purifying stevioside by recrystallization

A stevioside and recrystallization technology, applied in the field of food additives, can solve the problems affecting the normal development of the stevioside export industry and the like

Inactive Publication Date: 2011-12-21
LIAONING QIANQIAN BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the total glycoside content of stevioside produced in China is mostly 77%-85%, which is far from the requirements of the European Union and the United States, which affects the export of stevioside and the normal development of the industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 1kg stevioside, the total glucoside content is 65%-85%, put it into the crystallization container, then add 5L of methanol, heat and dissolve under the condition of stirring, heating temperature 60°C-65°C, keep it warm for 10 minutes, let it fully dissolve, Then add cooling water to slowly lower the temperature, the speed of cooling is reduced by 1°C-2°C every 10 minutes, and after falling to 10°C, keep warm for 24 hours to crystallize. After the crystallization was completed, the crystals and the mother liquor were separated by filtration, and 760 g of crystals were obtained after the crystals were dried. At this time, the content of stevioside was detected to be 90%. Repeat the above steps to recrystallize once to obtain 540g of pure stevioside, and the purity of stevioside is 98% after detection.

Embodiment 2

[0033] Take 1kg of stevioside, the total glycoside content is 65%-85%, and the content of rebaudioside (RA) in stevioside is higher than 50%, put it into the crystallization container, then add methanol 6L, heat and dissolve under the condition of stirring, Heating temperature is 60°C-65°C, keep it warm for 10 minutes, let it fully dissolve, then add cooling water to slowly cool down, the cooling speed is reduced by 1°C-2°C every 10 minutes, and keep it for 24 hours to crystallize after dropping to 5°C. After the crystallization was completed, centrifuged, and after the crystals were dried, 800 g of crystalline products were obtained. At this time, the content of stevioside was detected to be 89%. Repeat the above steps to recrystallize once to obtain 680g of pure stevioside, and the purity of stevioside is higher than 95% after testing.

Embodiment 3

[0035] Take 1kg of stevioside, the total glycoside content is 65%-85%, and the content of rebaudioside (RA) in stevioside is higher than 50%, put it into the crystallization container, then add methanol 7.5L, heat and dissolve under stirring condition , Heating temperature 60°C-65°C, keep it warm for 10 minutes, let it fully dissolve, then add cooling water to slowly cool down, the cooling speed will drop by 1°C-2°C every 10 minutes, and keep it for 48 hours to crystallize after dropping to 0°C. After the crystallization was completed, it was separated by pressure filtration. After the crystals were dried, 798g of crystals were obtained. At this time, the content of stevioside was detected to be 87%. Repeat the above steps to recrystallize once to obtain 680g of pure stevioside. The purity of stevioside is 95% after detection.

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Abstract

A method for purifying stevioside by recrystallization, the specific method is first heating and dissolving stevioside in 4-10 times the solvent under stirring, the heating temperature is ≤65°C, after it is completely dissolved, add cooling water to lower the temperature , lowered to 0°C-10°C for crystallization, the crystallization time is 12h-48h, the crystallization and mother liquor are separated by filtration, and then the crystals are crystallized again according to the crystallization conditions, and the purity of stevioside is higher than 95%. The invention is specially aimed at steviosides whose total glycosides are 65%-85%, and the method is simple and reasonable, easy to operate, and easy to form industrialized production.

Description

technical field [0001] The method for purifying stevioside by recrystallization belongs to the technical field of food additives. Background technique [0002] Stevioside is a kind of glycoside mixture composed of steviol, a tetracyclic diterpene compound, linked with different numbers of glycosides. At present, there are 12 kinds of substances found in the research, and there are eight kinds of substances that are recognized by people and have undergone medical toxicology experiments, specifically: [0003] (1) Stevioside (stevloside) (St) [0004] (2) Steviolbioside (steriolbioside) [0005] (3) Rebaudioside A (R-A) [0006] (4) Rebaudioside B (R-B) [0007] (5) Rebaudioside C (Rebaudioside C) (R-C) [0008] (6) Rebaudioside D (Rebaudioside D) (R-D) [0009] (7) Rebaudioside E (Rebaudioside E) (R-E) [0010] (8)Dulcoside A (dulcosiole A) (Dul-A) [0011] At present, the total stevioside content of stevioside produced in my country is 77%-85%. In terms of steviosid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/06
Inventor 郭玉孝孟庆华崔岩
Owner LIAONING QIANQIAN BIOLOGICAL TECH
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