Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for recovering rebaudioside A from primary crystallization mother liquor of steviol glycosides (SGs)

A technology of crystalline mother liquor and stevioside, which is applied in the field of separation of natural high-intensity sweeteners, can solve the problems of difficult recovery of rebaudioside A, and achieve the effect of simple and efficient recovery process, low energy consumption and good taste

Active Publication Date: 2020-09-15
SICHUAN UNIV
View PDF10 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In summary, since the RA content in the stevioside primary crystallization mother liquor is significantly lower than the St content (the RA content is about 1 / 2 to 2 / 3 of the St content), it is more difficult to recover rebaudioside A from the mother liquor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for recovering rebaudioside A from primary crystallization mother liquor of steviol glycosides (SGs)
  • Method for recovering rebaudioside A from primary crystallization mother liquor of steviol glycosides (SGs)
  • Method for recovering rebaudioside A from primary crystallization mother liquor of steviol glycosides (SGs)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Use a pipette to accurately measure 50ml of the mother liquor for primary crystallization and dry at 105°C to a constant weight to obtain a solid 6.168g, of which m RA =1874.5mg, m St =2982.7mg, RA / St=0.6284, and the ratio of RA peak area is 20.19%. Take another 50ml of the mother liquor of stevia crystallization, add dropwise 0.2g / ml potassium carbonate aqueous solution 2.0ml under stirring conditions at 25°C, continue stirring (n=100rpm) for 30min, centrifuge and filter and dry at 60°C for 24h to obtain a solid 1.809 g, where m RA =1322.0mg, m St =291.2mg, RA / St=4.54, the ratio of RA peak area is 60.62%, the recovery rate of RA is 70.5%, and the impurity content drops from 20.3% in the mother liquor to 14.4% (see attached Figure 2~4 )

Embodiment 2

[0035] Take 50ml primary crystallization mother liquor, add dropwise an appropriate concentration of potassium carbonate aqueous solution at different temperatures, stir for 30 minutes, and measure the content of each substance in the remaining mother liquor as shown in the table below.

[0036]

[0037]

Embodiment 3

[0039] Take 50ml primary crystallization mother liquor, add dropwise an appropriate concentration of potassium carbonate aqueous solution at 25°C, stir for 60min, and measure the content of each substance in the remaining mother liquor as shown in the table below.

[0040]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of separation of natural sweeteners. The solubility of each substance is changed by adding inorganic salt so as to selectively recover rebaudioside A (RA) in primarycrystallization mother liquor. The stevioside extracted from plants is a mixture and mainly contains rebaudioside A (RA), stevioside (ST), rebaudioside C (RC) and the like, wherein RA has the highestsweetness and is a primary crystallization product. The primary crystallization mother liquor mainly contains RA, ST, RC and some flavone and polyphenol impurities. According to the method, the crystallization characteristics and solubility of different components in the stevioside crystallization mother liquor are changed by utilizing the inorganic salt. An inorganic salt aqueous solution is directly added into the primary crystallization mother liquor and stirred, solids are separated out, filtered, washed and dried, and RA in the mother liquor can be recovered; and moreover, because part of impurities are removed, the taste is good. According to the technical method, equipment does not need to be increased, the operating steps are simple and short, and the energy consumption is low. RA, ST and RC are enriched in a liquid phase while most RA is recovered, and the substances are further separated and purified.

Description

Technical field [0001] The invention relates to the field of separation of natural high-intensity sweeteners, in particular to a method for recovering rebaudioside A from the mother liquor of primary crystallization of stevioside. Background technique [0002] Steviol glycosides (SGs) is a natural high-strength sweetener extracted from the natural plant Stevia. Many countries have approved stevioside as a food additive. Its sweetness is 200-300 times that of sucrose, and its calories are only sucrose. 1 / 300 of that. Natural stevia is a mixture consisting of several glycoside compounds. All steviol glycosides contain the same hydrophobic steviol glycoside unit. The difference is that the glycosyl substituents at C13 and C18 are different. The main component is rebaudio. Rebaudioside A (RA), Stevioside (St), and Rebaudioside C (Rebaudioside C, RC). The three of natural stevia account for more than 90%. Among them, RA has the highest sweetness, and its sweetness is similar to tha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/06C07H15/256
CPCC07H1/06C07H15/256
Inventor 宋航罗英杰顾建晖李景行姚舜兰先秋
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com