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Method for improving content of rebaudioside-A (RA), stevioside (ST), rebaudioside-C (RC) and/or total glucoside in stevia rebaudiana bertoni

A technology for steviol glycosides and stevia rebaudioside, which is applied in the field of increasing the content of rebaudioside A, steviol glycosides, rebaudioside C and/or total glycosides in stevia rebaudioside, and can solve the complex and high glycoside content of bioconversion stevioside. The problems of long time and high cost of stevia varieties

Active Publication Date: 2014-06-25
WILMAR SHANGHAI BIOTECH RES & DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods all have their defects. The method for cultivating high-glucoside stevioside varieties takes a long time, and the biotransformation stevioside technology is complicated and expensive.

Method used

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  • Method for improving content of rebaudioside-A (RA), stevioside (ST), rebaudioside-C (RC) and/or total glucoside in stevia rebaudiana bertoni
  • Method for improving content of rebaudioside-A (RA), stevioside (ST), rebaudioside-C (RC) and/or total glucoside in stevia rebaudiana bertoni
  • Method for improving content of rebaudioside-A (RA), stevioside (ST), rebaudioside-C (RC) and/or total glucoside in stevia rebaudiana bertoni

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1, jasmonic acid solution improves RA, ST and total glycoside content in Stevia rebaudiana plant leaf

[0061] 1.1. Stevia culture and growth

[0062] The seeds of the full-grained stevia variety "Huinong No. 1" were selected (purchased from Linquan County Wanquanfeng Agricultural Production Cooperative), and the stevia seeds were first sown in nutrient soil (the volume ratio of peat soil: vermiculite is 1:1) In the process, the seeds are covered with a thin layer of sand, germinate at 22°C, under the condition of 14 hours of light and 10 hours of darkness every day, and when the seedlings grow to 6-8 leaves, transplant the stevia seedlings into the cultivation pot (peat soil: vermiculite: perlite The volume ratio is 1:1:1), cultivated in 25°C, 14h light and 10h dark every day, watering and fertilizing regularly. After the Stevia rebaudiana grows to become seedlings, it is transferred to 25°C and grown under the condition of 12 hours of light and 12 hours of...

Embodiment 2

[0083] Embodiment 2, jasmonic acid acetone mixed solution improves RA, ST and total glycoside content in Stevia rebaudiana plant leaf

[0084] 2.1. Stevia Cultivation and Growth

[0085] Select the full-grained stevia variety "Fengnong No. 3" seeds (purchased from Xinnong Stevia Planting Professional Cooperative), and first sow the stevia seeds in nutrient soil (peat soil: vermiculite volume ratio is 1:1) , the seeds are covered with a thin layer of sand, germinate at 22°C, under 14h light and 10h dark conditions every day, and when the seedlings grow to 6-8 leaves, transplant the stevia seedlings into the cultivation pot (peat soil: vermiculite: perlite volume Ratio of 1:1:1), cultured at 25°C, 14h light and 10h dark every day, watered and fertilized regularly. After the Stevia rebaudiana grows to become seedlings, it is transferred to 25°C and grown under the condition of 12 hours of light and 12 hours of darkness every day for later use.

[0086] 2.2. Spraying the mixed s...

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Abstract

The invention discloses a method for improving the content of rebaudioside-A (RA), stevioside (ST), rebaudioside-C (RC) and / or total glucoside in stevia rebaudiana bertoni. The method comprises the step of spraying a mixed solution of jasmonic acid and jasmonic acid acetone on stevia rebaudiana bertoni plants before the step of harvesting the stevia rebaudiana leaves. According to the method, the content of the RA, the ST, the RC and / or the total glucoside in stevia rebaudiana bertoni leaves in the harvest period can be obviously improved.

Description

technical field [0001] The invention relates to a method for increasing the content of rebaudioside A, steviol glycoside, rebaudioside C and / or total glycosides in stevia rebaudiana. Background technique [0002] Stevia rebaudiana Bertoni (Stevia rebaudiana Bertoni), also known as "Stevia" and "Sweet Grass", is native to Paraguay, South America. It has been drunk as a sweet drink by local residents for centuries. It is a small perennial Compositae. Plants of the genus Via, perennial, fibrous, perennial, short-day, herbaceous. In 1970, Japan introduced stevia from Brazil, and began to domesticate, cultivate, and prepare glycosides. At the same time, it carried out tests such as toxicology and food testing, and first developed and utilized stevia products, steviol glycosides; Scientific research units such as the Chinese Academy of Agricultural Sciences have successfully introduced stevia from Japan for trial planting. Now Jiangsu, Fujian, Shandong, Xinjiang, Henan, Anhui an...

Claims

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Application Information

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IPC IPC(8): A01C21/00
Inventor 肖玲叶绍云史一鸣蔡南海许骏
Owner WILMAR SHANGHAI BIOTECH RES & DEV CENT
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