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Method for preparing rebaudioside C

A technology of recrystallization and ethanol, which is applied in the field of medicine, can solve problems such as low sweetness, heavy bitterness and astringent taste, and great influence on product quality, and achieve the effect of simple and easy method and improved purity

Active Publication Date: 2011-04-27
SHENYANG TIANFENG BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, rebaudioside A has the highest sweetness and the taste is closest to sucrose. Stevioside has the unique sweetness and coolness of stevioside products. Other ingredients not only have low sweetness, but also have different degrees of bitterness, although the content is not high. , but it has a great impact on the quality of the product, especially the bitter taste of rebaudioside C, which seriously affects the taste and quality of stevioside products, and has become an ingredient that manufacturers try to get out

Method used

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  • Method for preparing rebaudioside C
  • Method for preparing rebaudioside C
  • Method for preparing rebaudioside C

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 3Kg of stevioside (RC content is 3.5%, RA content is 38%, ST content is 33.58%), add 30L methanol to dissolve, heat and dissolve at 40°C, after cooling down to room temperature, put it in a water bath at 4°C and stir for crystallization for 24 hours , filtered, the crystallization mother liquor was decompressed and the solvent was recovered to obtain 1.52Kg of concentrate, which was set aside;

[0026] Take 20Kg of silica gel (200-300 mesh), add chloroform-92% ethanol (4:1) mixed solvent, fully remove air bubbles, put it into a chromatography column with a diameter of 273mm and a height of 1.5m; take another 1.0Kg of concentrate , add an appropriate amount of ethanol to dissolve, mix in 2.5Kg 200-300 mesh silica gel, and dry in an oven at 45°C for 24 hours; put the mixed silica gel into the chromatography column, and mix it with chloroform-92% ethanol (4:1) Solvent for elution, 10L / part of eluent collected, using TLC monitoring, TLC conditions: developer is n-butan...

Embodiment 2

[0028] Take 3Kg of stevioside ((RC content is 3.5%, RA content is 38%, ST content is 33.58%), add 24L methanol to dissolve, heat and dissolve at 40°C, after cooling down to room temperature, place it in a water bath at 4°C and stir to crystallize for 36 hour, filtered, and the crystallization mother liquor was decompressed and recovered solvent to obtain concentrate 1.56Kg, for subsequent use;

[0029] Take 25Kg of silica gel (200-300 mesh), add chloroform-92% ethanol (4:1) mixed solvent, fully remove the air bubbles, put it into a chromatography column with a diameter of 273mm and a height of 1.5m; take another 1.0Kg of concentrate , add an appropriate amount of ethanol to dissolve, mix in 2.5Kg 200-300 mesh silica gel, and dry in an oven at 45°C for 24 hours; put the mixed silica gel into the chromatography column, and mix it with chloroform-92% ethanol (4:1) Solvent for elution, 10L / part of eluent collected, using TLC monitoring, TLC conditions: developer is n-butanol-glaci...

Embodiment 3

[0031] Take 3Kg of stevioside (RC content is 10.80%, RA content is 37.14%, ST content is 35.53%), add 36L methanol to dissolve, heat and dissolve at 40°C, after cooling down to room temperature, put it in a water bath at 4°C and stir for crystallization for 48 hours , filtered, the crystallization mother liquor was decompressed and the solvent was recovered to obtain 1.54Kg of concentrate, which was set aside;

[0032] Take 30Kg of silica gel (200-300 mesh), add chloroform-95% ethanol (3:1) mixed solvent, fully remove air bubbles, put it into a chromatography column with a diameter of 273mm and a height of 1.5m; take another 1.0Kg of concentrate , add an appropriate amount of ethanol to dissolve, mix in 2.5Kg 200-300 mesh silica gel, and dry in an oven at 45°C for 24 hours; put the mixed silica gel into the chromatography column, and mix it with chloroform-95% ethanol (3:1) Solvent for elution, 10L / part of eluent collected, using TLC monitoring, TLC conditions: developer is n-...

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Abstract

The invention relates to a method for preparing high-purity stevioside rebaudioside RC, and belongs to the technical field of food additives. In the method, stevioside is used as a raw material, rebaudioside RC content in the raw material is about between 3 and 15 percent, and recrystallization technology and column chromatography technology are combined. The method comprises the following steps of: performing recrystallization by dissolving the stevioside which serves as the raw material in methanol; stirring and crystallizing at the temperature of 4 DEG C for 24 to 48 hours; recovering a solvent from crystallization mother liquor under reduced pressure to obtain a concentrate; performing silica gel column chromatography on the concentrate under the conditions that a stationary phase is silica gel with particle size of between 200 and 300 meshes, the weight of the silica gel mixed with a sample is 2.5 times that of the concentrate and the mass of pure silica gel is 20 to 50 times that of the concentrate; performing elution by using a mixed solvent system of trichloromethane and ethanol in the ratio of 2 to 1 or dichloromethane and ethanol in the ratio of 2 to 1, wherein water content in the ethanol is 2 to 10 percent; and collecting rebaudioside RC-containing main section eluent, mixing, concentrating, drying, detecting and packaging. By the method, a rebaudioside C product with purity of more than 95 percent can be obtained; and the method has a simple process and is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a preparation method of Rebaudioside C (Rebaudioside C), in particular to a method for separating high-purity Rebaudioside C from stevioside. Background technique [0002] The sweet ingredients in stevia are all glycosides. Glycoside (glycoside or heteroside), also known as glycoside or glycoside, is a compound formed by the condensation of sugar or a sugar derivative and another non-sugar compound through the carbon atom of the sugar terminal group. Glycosides can be hydrolyzed to produce two parts, sugar and non-sugar. The non-sugar part is called aglycon or aglycone, glycoside (agly-cone, genin). Foreign studies have isolated 8 different sweeteners from stevia leaf extracts. Taste components: namely stevioside (stevioside); rebaudioside (renaudiside) A, B, C, D, E; dulcoside (dulcoside) A, B, these 8 ingredients are based on the same diterpene ligand The formed glycosides bel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/24C07H1/08
Inventor 王继文李英娜王文雷茂林张春婷朱兵兵
Owner SHENYANG TIANFENG BIOLOGICAL PHARMA
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