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Technology for purifying rebaudioside C

A rebaudioside and process technology, applied in the field of extraction and purification of active ingredients in plants, can solve the problem of low RC content of products, and achieve the effects of increasing RC content and yield, increasing yield, increasing content and yield

Active Publication Date: 2014-05-21
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method is simple to operate, but the RC content in the obtained product is not high

Method used

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  • Technology for purifying rebaudioside C
  • Technology for purifying rebaudioside C
  • Technology for purifying rebaudioside C

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1) Take 300g of crude stevia extract dry powder with RC content of 10%, crystallize with 60v / v% methanol of 4 times the amount of the raw material, and then put the mother liquor into the D101 macroporous resin column, first use 1w / w of 2 times the volume of the column bed % sodium hydroxide aqueous solution to wash the column, then wash the column with 2 times the column bed volume of 1w / w% hydrochloric acid aqueous solution, then wash the resin column with water until the effluent is neutral, and then use 2.5 times the column bed volume to pH 3 20v / v% ethanol (adjust the pH value with formic acid) for elution, and finally elute with 60v / v% ethanol (adjust the pH value with formic acid) at pH 7.5, track and detect with thin layer chromatography, and collect 60v / v% ethanol The eluate was concentrated and dried to obtain 70 g of the crude product of rebaudioside C; the total stevioside content in the obtained crude product was 91%, wherein the RC content was 40.5%, and th...

Embodiment 2

[0041] 1) Take 400g of thick stevia extract dry powder with RC content of 11%, crystallize with 70v / v% ethanol of 4 times the amount of the raw material, and then enter the mother liquor into the LX-8 macroporous resin column, first use 0.5 Wash the column with w / w% sodium hydroxide aqueous solution, then wash the column with 1w / w% sulfuric acid aqueous solution of 2 times the column bed volume, then wash the resin column with water until the effluent is neutral, and then use 2.9 times the column bed volume Elute with 25v / v% ethanol at pH 3.5 (adjust the pH value with glacial acetic acid), and finally elute with 70v / v% ethanol at pH 8.0 (adjust the pH value with glacial acetic acid), track and detect with thin layer chromatography, and collect The 70v / v% ethanol eluate was concentrated and dried to obtain 90g of a crude product of rebaudioside C; the content of total steviosides in the obtained crude product was 93%, wherein the content of RC was 41.5%, and the content of RA wa...

Embodiment 3

[0044] 1) Take 350g of thick stevia extract dry powder with RC content of 13%, crystallize with 75v / v% acetone of 4 times the amount of the raw material, and then enter the mother liquor into the HP-20 macroporous resin column, first use 1.5 Wash the column with w / w% potassium hydroxide aqueous solution, then wash the column with 3 times the column bed volume of 1w / w% hydrochloric acid aqueous solution, then wash the resin column with water until the effluent is neutral, and then use 3 times the column bed volume Elute with 28v / v% ethanol at pH 4 (adjust the pH with glacial acetic acid), and finally elute with 65v / v% ethanol at pH 7.5 (adjust the pH with glacial acetic acid). The 65v / v% ethanol eluate was concentrated and dried to obtain 103g of a crude product of rebaudioside C; the content of total steviol glycosides in the crude product was 94%, of which RC content was 43% and RA content was 33%;

[0045] 2) Add the rebaudioside C crude product obtained above into a crystal...

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Abstract

The invention discloses a technology for purifying rebaudioside C. The technology comprises the following steps: 1) crystallizing a coarse stevia extract with a solvent A, loading a mother liquor on a macroporous resin column, washing the column by using an alkali liquor and an acid liquor firstly, and then washing the column by using water until an effluent is neutral, eluting by using 10-30v / v% ethanol of which the pH is 3-5; finally, eluting by using 60-85v / v% ethanol of which the pH is 7-8, collecting 60-85v / v% ethanol eluant, concentrating and drying to obtain a crude product of the rebaudioside C; 2) dissolving the crude product of the rebaudioside C with a solvent B, and adjusting the pH to 3-5 by using an acid, devitrifying under the condition of 2-10 DEG C, collecting crystals, washing to neutral and drying, so as obtain the rebaudioside C. By adopting the technology disclosed by the invention, the product of which the RC content is higher than 97% can be obtained just by primary crystallization, the RC overall recovery rate can be higher than 75%, and the technology is simple and easy to operate, and beneficial to industrial production.

Description

technical field [0001] The invention relates to the technical field of extraction and purification of active ingredients in plants, in particular to a purification process of rebaudioside C. Background technique [0002] Stevioside is a new type of natural sweetener extracted from the Compositae herb stevia (or stevia leaf). It has the characteristics of high sweetness and low calorie. Its sweetness is 200-300 times that of sucrose. The calorific value is only 1 / 300 of that of sucrose. A large number of scientific experiments and long-term consumption history in other countries have proved that stevioside is non-toxic, has no side effects, no carcinogens, and is safe to eat. It is an ideal sweetener that can replace sucrose; and South America uses stevia as a herb and sugar substitute. There are hundreds of years of history. Information from the international sweetener industry shows that steviol glycosides have been widely used in the production of food, beverages, and se...

Claims

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Application Information

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IPC IPC(8): C07H15/24C07H1/08
Inventor 赵军杨文国宋云飞李元元李宇钰
Owner GUILIN NATURAL INGREDIENTS CORP
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