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Method for purifying rebaudioside c

一种乙酸乙酯、丁醇的技术，应用在化学仪器和方法、有机化学、糖衍生物等方向，能够解决产率物质纯度未公开等问题

Inactive Publication Date: 2013-05-29
INTERNATIONAL FLAVORS & FRAGRANCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantage of this method is that high purity rebaudioside C is required to induce purification
In addition, the yield of the method and the purity of the material obtained are not disclosed

Method used

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  • Method for purifying rebaudioside c
  • Method for purifying rebaudioside c

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1; Initial Purification of Rebaudioside C from Crude Extract

[0035] Using a number of different solvent systems, a mixture of acetone and water was observed to be a suitable solvent for the recrystallization of rebaudioside C from the crude starting material (i.e., the "waste" produced in the rebaudioside A production process) . Therefore, the yield of recrystallized rebaudioside C from the starting material was determined. For these experiments, the crude starting material was mixed with acetone / water (85 / 15, w / w). Typically, for 1 g of solid, 4-6 g of solvent is used. The mixture was heated to 48°C and all solids were allowed to dissolve. The mixture was cooled to room temperature and stirred for 3-4 days. The solution was filtered to collect a white precipitate, which was then washed with acetone and dried under vacuum at 50°C for 8 hours.

[0036] Table 2

[0037] Amount of crude starting material

RebC content in crude product

The am...

Embodiment 2

[0047] Example 2: Purification of Rebaudioside C

[0048] 20 g of starting material are dissolved in 500 ml of a THF / water mixture containing 70-80% (preferably 75% (v / v)) water. The resulting solution was extracted twice with 500 ml of ethyl acetate / 1-butanol mixture (85 / 15, v / v). The 1-butanol / ethyl acetate layer was removed.

[0049] The THF-water phase is then further extracted twice with 500 ml of an ethyl acetate / butanol mixture containing 30-40% 1-butanol, preferably 35% (v / v). The ethyl acetate / butanol layers from the two extractions were combined and concentrated under vacuum to give 8.70 g of material. It was determined by HPLC analysis that the content of rebaudioside C in this substance reached 24.5% (Table 5).

[0050] table 5

[0051] Retention time (minutes)

components

total percentage

12.702

unknown

1.10

15.722

unknown

4.62

18.547

unknown

4.20

22.811

unknown

1.06

24.473

...

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PUM

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Abstract

The present invention is a method for purifying Rebaudioside C by subjecting 'waste material', generated during the Rebaudioside A manufacturing process, to liquid-liquid extraction and recrystallizing the Rebaudioside C.

Description

Background technique [0001] South American perennial Stevia (Stevia rebaudiana Bertoni) is known for its use as a natural sweetener. The sweet taste of the stevia plant is due to the presence of highly sweet sweet glucosides. Glycosides derived from stevia (steviol glycosides) include stevioside (4-13% dry weight), rebaudioside (Rebaudioside) A (2-4%), rebaudioside C (1-2%), dur Dulcoside A (0.4-0.7%) and trace amounts of steviolbioside, rubusoside and rebaudioside B, D, E and F (Kinghorn (2002) Kinghorn (ed.) Stevia, Medicinal and Aromatic Plants-Industrial Profiles, Volume 19 (Taylor and Francis, London and NY). Of these glycosides, only stevioside and rebaudioside A are currently commercially available in large quantities. The raw material for the manufacture of stevioside and rebaudioside A is the crushed leaves of perennial shrubs. Conventional extraction methods for obtaining steviol glycoside-containing extracts are disclosed in literature following similar procedur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/06
CPCC07H1/08C07H15/256C07H1/06
Inventor Z·陈
Owner INTERNATIONAL FLAVORS & FRAGRANCES
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