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Method for preparing rebaudioside C from stevia sugar

A technology of stevia and silica gel, applied in the field of medicine and food

Inactive Publication Date: 2014-08-06
SHENYANG TIANFENG BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, ordinary screening screens and distribution plates are mostly circular or mesh structures, which cannot solve the problem of uniform distribution of chromatographic bands in the horizontal direction.

Method used

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  • Method for preparing rebaudioside C from stevia sugar
  • Method for preparing rebaudioside C from stevia sugar
  • Method for preparing rebaudioside C from stevia sugar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]Take 1.5Kg of stevioside (RC content is 15.23%), add an appropriate amount of methanol to dissolve, mix in 3.75Kg of 200-300 mesh silica gel, and dry in an oven at 45°C for 24 hours; put the mixed silica gel into the high-pressure chromatography column, and tap it And keep the sample loading silica gel surface parallel to the round surface of the column top; put 22.5kg silica gel (10-40μm) into the high-pressure chromatography column on the top of the sample mixing silica gel, compress the silica gel column bed axially, and the upward stroke of the piston is 280±5mm ; Add dichloromethane to the column to balance the column bed. After the balance is good, change to dichloromethane-90% ethanol (2:1) for elution, collect 10L / part of the eluent, and use TLC monitoring. The TLC conditions are: The developer is n-butanol-glacial acetic acid-ether-water, the stationary phase is GF254 silica gel, and the developer is 50% sulfuric acid-water solution. Merge according to the resul...

Embodiment 2

[0035] Take 1.5Kg of stevioside (with RC content of 25.38%), add appropriate amount of methanol to dissolve, mix in 3.75Kg of 200-300 mesh silica gel, and dry in an oven at 45°C for 24 hours; put the mixed silica gel into the high-pressure chromatography column, and tap it And keep the sample silica gel surface parallel to the round surface of the column top; put 24kg silica gel (10-40μm) into the high-pressure chromatography column above the sample mixing silica gel, compress the silica gel column bed axially, and the upward stroke of the piston is 280±5mm; Add dichloromethane to the column to balance the column bed. After the balance is good, change to dichloromethane-98% methanol (2.5:1) for elution, collect 10L / part of the eluate, and use TLC monitoring. The TLC condition is: Expand The reagent is n-butanol-glacial acetic acid-ether-water, the stationary phase is GF254 silica gel, and the color developer is 50% sulfuric acid-water solution. Merge according to the results o...

Embodiment 3

[0037] Take 1.5Kg of stevioside (RC content is 40.25%), add an appropriate amount of methanol to dissolve, mix in 3.75Kg of 200-300 mesh silica gel, and dry in an oven at 45°C for 24 hours; put the mixed silica gel into the high-pressure chromatography column, and tap it And keep the sample loading silica gel surface parallel to the round surface of the column top; put 26kg of silica gel (10-40μm) into the high-pressure chromatography column on the top of the sample mixing silica gel, compress the silica gel column bed axially, and the upward stroke of the piston is 280±5mm; Add chloroform to the column to balance the column bed. After the balance is good, change to chloroform-92% methanol (3:1) for elution, collect 10L / part of the eluate, and use TLC monitoring. The TLC condition is: Expand The reagent is n-butanol-glacial acetic acid-ether-water, the stationary phase is GF254 silica gel, and the color developer is 50% sulfuric acid-water solution. Merge according to the resu...

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Abstract

The invention belongs to the technical field of medicine food, and relates to a preparation method for separating rebaudioside C from stevia sugar, specifically to a method for preparing rebaudioside C by applying an axial direction high pressure preparation chromatography technology. The method specifically comprises: taking stevia sugar, adding methanol to dissolve, adding silica gel and stirring, drying, loading the silica gel mixed with a sample and a blank silica gel into a chromatographic column to carry out chromatography, loading the silica gel mixed with the sample in a high pressure chromatographic column, axially compressing the chromatographic column bed, rinsing the column to balance the column bed, adding a trichloromethane or dichloromethane and water-containing alcohol mixed solvent to elute after completing the balance, collecting the elution solution, adopting thin-layer monitoring, mixing the rebaudioside C main section, and carrying out reduced pressure concentration, drying and detection to obtain the high purity rebaudioside. Compared with the open type chromatographic column, the method of the present invention is efficient, fast, simple and easy-performing and is suitable for industrial production, wherein the prepared product has high purity.

Description

technical field [0001] The invention belongs to the technical field of medicine and food, and relates to a preparation method for separating rebaudioside C (Rebaudioside C) from stevioside, in particular to a method for preparing rebaudioside C by applying an axial high-pressure preparative chromatographic technique. Background technique [0002] The sweet ingredients in stevia are all glycosides. Glycosides, also known as glycosides or glycosides, are compounds formed by the condensation of sugar or sugar derivatives and another non-sugar compound through the connection and condensation of sugar terminal carbon atoms. Glycosides can be hydrolyzed to produce two parts, sugar and non-sugar. The non-sugar part is called aglycon or aglycone, glycoside (agly-cone, genin). Foreign studies have isolated 8 different sweeteners from stevia leaf extracts. Taste components: namely stevioside (stevioside); rebaudioside (renaudiside) A, B, C, D, E; dulcoside (dulcoside) A, B, these 8 i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/06
Inventor 王继文李英娜苏宏伟刘涛王莹
Owner SHENYANG TIANFENG BIOLOGICAL PHARMA
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