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Method for reaction separation purification of rebaudioside A

A technology of reaction and solid-liquid separation, applied in the field of mechanochemistry, can solve problems such as poor specificity, and achieve the effects of reducing pollution, wide application, and simple process

Active Publication Date: 2015-06-24
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inorganic acid-base catalyzed hydrolysis has poor specificity for steveside and rebaudioside A
And different glycosidases can catalyze the hydrolysis of steveside and rebaudioside A into various products (see appendix figure 1 ), but at the same time can hydrolyze the two different glucose residues of C19 and C13 in the aglycone, and can specifically catalyze the hydrolysis of steveside and rebaudioside A through the unique affinity relationship. one with less glycosidase

Method used

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  • Method for reaction separation purification of rebaudioside A
  • Method for reaction separation purification of rebaudioside A
  • Method for reaction separation purification of rebaudioside A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Mix the stevioside crude extract (80% stevioside, 10% rebaudioside A, and the remaining 5% are RC and other impurities) and water at a mass ratio of 1:10, heat and dissolve under stirring until all steviosides are dissolved Afterwards, preheat at 75°C for 30 minutes and add β-galactosidase 600 U / g steveside from Sulfolobus solfataricus to react for 16 hours. The reaction precipitate and the reaction solution are separated into solid and liquid by using a high-speed centrifuge, and the obtained The solid was recrystallized twice with water, and rebaudioside A was precipitated from the water, and dried to obtain rebaudioside A with a purity of 95.1% and a yield of 90.3%. .

Embodiment 2

[0018] Mix the stevioside crude extract (50% stevioside, 46% rebaudioside A, and the remaining 4% are RC and other impurities) and water at a mass ratio of 1:15, heat to dissolve under stirring, and wait until the stevioside is completely dissolved Afterwards, preheat at 75°C for 30 minutes and add β-galactosidase 600 U / g steveside from Sulfolobus solfataricus to react for 16 hours. The reaction precipitate and the reaction solution are separated into solid and liquid by using a high-speed centrifuge, and the obtained The solid was recrystallized twice with water, rebaudioside A was precipitated from the water, and dried to obtain rebaudioside A with a purity of 95.6% and a yield of 90.8%.

Embodiment 3

[0020] Mix the stevioside crude extract (55% stevioside, 35% rebaudioside A, and the remaining 5% is RC and other water-soluble impurities) and water at a mass ratio of 1:10, heat and dissolve under stirring, and wait until the stevioside After all dissolved, add β-galactosidase 600U / g steveside from Sulfolobus solfataricus and react for 16 hours at 75°C preheating for 30 minutes, and separate the reaction precipitate from the reaction liquid by using a high-speed centrifuge for solid-liquid separation. The obtained solid was recrystallized twice with water, and rebaudioside A was precipitated from the water, and dried to obtain rebaudioside A with a purity of 95.5% and a yield of 90.7%.

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Abstract

The present invention discloses a method for reaction separation purification of rebaudioside A, and belongs to the technical field of organic chemistry. In the prior art, the stevia sugar crude product mainly contains stevioside, high-quality sweeteners such as rebaudioside A and rebaudioside C, and small amounts of other steviol derivatives, the rebaudioside A and the stevioside have the similar structure, and the dissolubility of the two substances can be increased when the two substances co-exist in a polar solution, such that the multi-stage sectional organic solvent re-crystallization is required to obtain the high-purity rebaudioside A. According to the present invention, the beta-galactosidase having lipase activity and galactosidase activity and derived from sulfolobus solfataricus is utilized to carry out highly specific catalytic hydrolysis on the stevioside, such that the poor-quality stevioside can be subjected to complete catalysis conversion into the water-insoluble steviol, the rebaudioside A and other steviols can not be hydrolyzed so as to remain in the solution and the high-purity rebaudioside A can be obtained through the simple aqueous phase re-crystallization, and the by-produced steviol is the organic synthesis intermediate having the wide use and the added value of higher than the stevioside.

Description

【Technical field】 [0001] The invention belongs to the technical field of mechanochemistry, and in particular relates to a method for separating and purifying rebaudioside A through reaction. 【Background technique】 [0002] Stevioside is a kind of low-calorie high-power sweetener extracted from stevia leaves. Its sweetness is 200-350 times that of sucrose, but its calories are only 1 / 300 of sucrose, so it is a very ideal Sweeteners that replace sucrose. Stevioside (stevioside) and rebaudioside A (Rebaudioside A) are the two substances with the most content in stevioside, and rebaudioside A is the compound with the best sweetness properties in stevioside, which is the only one recognized by FDA "Generally safe" (GRAS) substance; however, stevioside has a bad aftertaste and is used at a low value. The structural formulas of steveside and rebaudioside A are as follows: [0003] [0004] After stevioside and rebaudioside A hydrolyze their C19 and C13 glycosidic bonds and es...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/08C12P7/42
CPCC07H1/06C07H15/256C12P7/42
Inventor 方云陈俊名夏咏梅
Owner JIANGNAN UNIV
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