Method for obtaining high-purity stevioside and enriching rebaudioside C from primary crystallization mother liquor of stevioside through secondary crystallization

A technology for crystallizing mother liquor and stevia, which is applied in the field of separation of natural high-intensity sweeteners, and achieves the effects of simple and efficient process and low energy consumption

Pending Publication Date: 2021-08-03
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Liu Yaxian et al. (Liu Y, Hua X, Wang M, etal.Purification of the mother liquor sugar from induSTrial STevia productionthrough one-STep adsorption by non-polar macroporous resin[J]. Food ChemiSTr...

Method used

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  • Method for obtaining high-purity stevioside and enriching rebaudioside C from primary crystallization mother liquor of stevioside through secondary crystallization
  • Method for obtaining high-purity stevioside and enriching rebaudioside C from primary crystallization mother liquor of stevioside through secondary crystallization
  • Method for obtaining high-purity stevioside and enriching rebaudioside C from primary crystallization mother liquor of stevioside through secondary crystallization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Precisely measure 100 mL of primary crystallization mother liquor with a pipette, and dry at 100°C to constant weight to obtain a solid. Take 2g of solid for later use, configure 90% methanol and ethanol aqueous solution as the crystallization solvent, put it into a beaker with a solid-to-liquid ratio of 1:8 (g / mL) and stir for 30 minutes, let it stand at 25°C for natural crystallization for 8 hours, and filter the solid for Pure methanol was washed, dried and weighed, and samples were taken for HPLC analysis according to the national standard method.

Embodiment 2～21

[0032] Examples 2-21 of crystallization under different conditions applied to the recovery of high-purity ST are shown in the table below. The parameter condition not mentioned in the form is identical with embodiment 1.

[0033]

[0034]

[0035] In summary, the present invention provides a method for recovering stevioside ST from the primary crystallization mother liquor of industrial stevioside. Utilizing the influence of the crystallization solvent added with isopropanol on the solubility of various substances in stevioside, and selectively recovering ST, this method is simple and efficient, convenient and quick, and removes a part of flavonoid bitter impurities, which provides a basis for the subsequent preparation of high-purity rebaudioside C raw materials.

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Abstract

The invention relates to the field of separation of natural high-potency sweeteners. The solubility of each component in stevioside is changed by selecting a crystallization solvent so as to selectively recover stevioside in primary crystallization mother liquor. Stevioside extracted from plants is a mixture and mainly contains rebaudioside A (RA), stevioside (ST), rebaudioside C (RC) and the like, and high-purity RA can be obtained through one-time crystallization. The remaining primary crystallization mother liquor mainly contains ST, RA, RC and some bitter impurities such as flavones and polyphenols. The ST content is maximum. According to the method, the solubility difference of different components in the stevioside crystallization mother liquor in an improved crystallization solvent is utilized. When mother liquor sugar is subjected to secondary crystallization, solid is separated out and then filtered, washed and dried, and ST in the mother liquor can be recycled. According to the technical method, equipment does not need to be added, operation steps are simple, and energy consumption is low. And when most of ST is recycled, RC is enriched in a liquid phase, so that the substances are further separated and purified.

Description

technical field [0001] The invention relates to the field of separation of natural high-intensity sweeteners, in particular to a method for obtaining high-purity stevioside and enriching rebaudioside C from a stevioside primary crystallization mother liquor through secondary crystallization. Background technique [0002] Stevia sugar (STeviol glycosides, SGs) is a natural high-power sweetener extracted from the natural plant Stevia rebaudiana. Many countries have approved stevia sugar as a food additive. Its sweetness is 200-300 times that of sucrose, and its calories are only sucrose. 1 / 300 of. Natural stevioside is a mixture consisting of several glycoside compounds. Steviosides all contain the same hydrophobic stevioside unit. The difference is that the glycosyl substituents at the C13 and C18 positions are different. The main component is Leba Diglycoside A (Rebaudioside A, RA), stevioside (STevioside, ST), rebaudioside C (Rebaudioside C, RC). The three together accoun...

Claims

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Application Information

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IPC IPC(8): C07H1/06C07H15/256
CPCC07H1/06C07H15/256
Inventor 宋航罗英杰顾建晖李福林姚舜杨赵梓源
Owner SICHUAN UNIV
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